A procedure for the quantification of steroid hormones of various classes in human urine (androgens, estrogens, progestins, corticosteroids) has been described consisting of sample preparation by means of dispersive liquid-liquid extraction after enzymatic hydrolysis with β-glucuronidase from E. Coli followed by ultra-high performance liquid chromatography–high resolution mass spectrometry (quadrupole time-of-flight) detection. Both one-variable-at-a-time and multivariate approaches (full factorial and Box-Behnken designs) were applied to optimize sample preparation conditions. The procedure was validated using synthetic urine in the concentration range of 0.25–500 ng/mL. Then, it was applied to the analysis of real urine samples and the results were compared with those of a common liquid-liquid extraction procedure. The results obtained proved its applicability to the quantification of steroid hormones in human urine with high sensitivity and accuracy.

已经描述了定量人类尿液中各种类别的类固醇激素（雄激素，雌激素，孕激素，皮质类固醇）的方法，该方法包括在用来自大肠杆菌的β-葡糖醛酸苷酶进行酶水解后，通过分散液-液萃取制备样品。然后进行超高效液相色谱-高分辨率质谱（四极杆飞行时间）检测。一次使用一次变量和多变量方法（全因子和Box-Behnken设计）都可以优化样品制备条件。使用合成尿液在0.25–500 ng / mL的浓度范围内对该程序进行了验证。然后，将其用于实际尿液样品的分析，并将结果与​​普通液-液提取程序的结果进行比较。所获得的结果证明了其在人类尿液中类固醇激素的定量分析中具有很高的灵敏度和准确性。