This research was carried out in a tissue culture study to analyze the impact of silicon dioxide nanoparticles on growth, photosynthesis and protective enzymes of a bamboo species (Arundinaria pygmaea) under three various heavy metal stresses (copper (Cu), manganese (Mn), and cadmium (Cd)). The treatments consisted of four concentrations of heavy metals (50, 100, 200, 400 μM) in combination with 100 μM silicon dioxide nanoparticles in comparison to controls. The results indicated that silicon dioxide nanoparticles under heavy metals (Cu and Mn) increased plant growth relative to the control, and they alleviated Cu and Mn toxicity, as reflected by significant increases in protective enzymes, chlorophyll content and fluorescence, as well as plant biomass and shoot length. However, the treatments under Cd concentrations resulted in a significantly decreased biomass and shoots length, which suggested that 100 μM silicon dioxide nanoparticles treatment was unable to prevent Cd accumulation in leaves through internal and external mechanisms. Overall, we concluded that 100 μM silicon dioxide nanoparticles increased the tolerance of bamboo species to heavy metals stress (Cu and Mn) and promoted plant growth indexes by evoking key mechanisms, including inhibiting metal accumulation in the leaves by the adsorption of the metal ions onto silicon dioxide nanoparticles, increasing levels of protective enzymes, and improving photosynthetic properties such as enhanced light-use efficiency and light transmission. We suggest that the combination of 100 μM silicon dioxide nanoparticles with concentrations of 100 and 200 μM heavy metals (Cu and Mn) can confer optimal growth rate, representing the threshold of the plant tolerance to heavy metals in a combination with 100 μM silicon dioxide nanoparticles.

这项研究是在组织培养研究中进行的，目的是分析二氧化硅纳米颗粒对竹子（Arundinaria pygmaea）的生长，光合作用和保护酶的影响。）在三种不同的重金属应力下（铜（Cu），锰（Mn）和镉（Cd））。与对照相比，处理包括四种浓度的重金属（50、100、200、400μM）与100μM二氧化硅纳米颗粒的组合。结果表明，重金属（铜和锰）下的二氧化硅纳米粒子相对于对照提高了植物的生长，并且减轻了铜和锰的毒性，这通过保护性酶，叶绿素含量和荧光以及植物生物量的显着增加得以体现。和拍摄长度。然而，在Cd浓度下进行处理导致生物量和枝条长度显着减少，这表明100μM二氧化硅纳米粒子处理无法通过内部和外部机制阻止Cd在叶片中的积累。总体，我们得出的结论是，100μM的二氧化硅纳米颗粒通过激发关键机制提高了竹种对重金属胁迫（铜和锰）的耐受性，并促进了植物的生长指数，包括通过将金属离子吸附到二氧化硅上来抑制叶片中的金属积累纳米颗粒，增加保护酶的水平以及改善光合特性，例如提高光利用效率和透光率。我们建议将100μM二氧化硅纳米粒子与100和200μM重金属（铜和锰）的浓度结合使用可赋予最佳生长速率，这代表了与100μM二氧化硅纳米粒子结合使用时植物对重金属的耐受性阈值。