Fungi are a highly diverse group of organisms and play a significant role in decomposition and carbon cycling in boreal ecosystems. To determine how fungal communities are structured in peat bogs and how to obtain representative samples for monitoring fungal community changes, we separately sampled and sequenced (ITS2, Illumina MiSeq) peat, mixed litter and litter from individual dominant plant species in five permanent plots in raised bogs in the Jizerské Hory Mountains (Czech Republic). In total, we detected 68–103 OTUs per plot. The fungal assemblages were mostly influenced by substrate identity, whereas the effect of the site was minimal. Only a few identified OTUs behave like generalists. The most specific fungi were found in dead parts of Calluna vulgaris, Trichophorum cespitosum and Drosera rotundifolia. The high substrate heterogeneity means that sequencing soil core samples does not provide an exhaustive inventory of the fungal diversity in bogs. Poor correspondence was observed between the recorded OTUs and either visually inspected fruitbodies or literature reports on the fungal taxa associated with the analysed substrates.

真菌是高度多样化的生物体，在北方生态系统的分解和碳循环中起重要作用。为了确定泥炭沼泽中真菌群落的结构以及如何获取代表性样品以监测真菌群落变化，我们分别对五个永久性样地中的主要优势植物物种的泥炭，混合凋落物和凋落物进行了采样和测序（ITS2，Illumina MiSeq）。 JizerskéHory Mountains（捷克共和国）的沼泽。总体而言，我们每个地块检测到68-103个OTU。真菌组合主要受底物同一性的影响，而该位点的影响很小。只有少数已确定的OTU表现像通才。最常见的真菌是在寻常的Calluna，Trichophorum cespitosum和罗氏沼虾。高的底物异质性意味着对土壤核心样品进行测序无法提供沼泽中真菌多样性的详尽清单。在记录的OTU与肉眼检查的果体或与分析底物相关的真菌类群的文献报告之间观察到差的对应性。