To study the homogeneity and heterogeneity of CD4⁺CD25⁺ T cells receptor β-chain complementarity determining region 3 (TCR β CDR3) repertoires in breast tumor tissues, lung metastatic tissues, and spleens from 4T1 tumor-bearing BALB/c mice. We used high-throughput sequencing to analyze the characteristics and changes of CD4⁺CD25⁺ TCR β CDR3 repertoires among tumor tissues, lung metastatic tissues, and spleens. The diversity of the CD4⁺CD25⁺ TCR β CDR3 repertoires in breast tumor tissue was similar to that of lung metastatic tissues and less pronounced than that of spleen tissues. Breast tumor tissues and lung metastatic tissues had a greater number of high-frequency CDR3 sequences and intermediate-frequency CDR3 sequences than those of spleens. The proportion of unique productive CDR3 sequences in breast tumor tissues and lung metastatic tissues was significantly greater than that in the spleens. The diversity and frequency of the CDR3 repertoires remained homogeneous in breast tumors and lung metastatic tissues and showed great heterogeneity in the spleens, which suggested that the breast tissues and lung metastatic tissues have characteristics of CD4⁺CD25⁺ T cells that relate to the tumor microenvironment. However, the number and characteristics of overlapping CDR3 sequences suggested that there were some different CD4⁺CD25⁺ T cells in tumors and in the circulatory immune system. The study may be used to further explore the characteristics of the CDR3 repertoires and determine the source of the CD4⁺CD25⁺ T cells in the breast cancer microenvironment.

中文翻译：

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4T1荷瘤BALB / c小鼠的乳腺肿瘤组织，肺转移组织和脾脏中CD4 + CD25 + T细胞TCRβCDR3组成成分的异质性分析

为了研究CD4的均匀性和非均质性⁺ CD25 ⁺ T细胞受体β α链互补决定区3（TCR β CDR3）在乳腺肿瘤组织，肺转移组织，和脾全集从荷有4T1肿瘤的BALB / c小鼠。我们使用高通量测序分析的特点和CD4的变化⁺ CD25 ⁺ TCR β肿瘤组织，肺转移组织，和脾中CDR3全集。在CD4的多样性⁺ CD25 ⁺ T细胞受体β乳腺肿瘤组织中的CDR3组成与肺转移组织相似，但不如脾组织那么明显。乳腺肿瘤组织和肺转移组织比脾脏具有更多的高频CDR3序列和中频CDR3序列。乳腺肿瘤组织和肺转移组织中独特的生产性CDR3序列的比例显着大于脾脏。CDR3组成成分的多样性和频率在乳腺肿瘤和肺转移组织中保持均一性，并且在脾脏中表现出很大的异质性，这表明乳腺组织和肺转移组织具有CD4 ⁺ CD25 ^+的特征。与肿瘤微环境有关的T细胞。然而，重叠的CDR3序列的数量和特征表明，肿瘤和循环免疫系统中存在一些不同的CD4 ⁺ CD25 ⁺ T细胞。该研究可用于进一步探索CDR3库的特征，并确定乳腺癌微环境中CD4 ⁺ CD25 ⁺ T细胞的来源。