We demonstrate diffraction-limited and super-resolution imaging through thick layers (tens-hundreds of microns) of BIO-133, a biocompatible, UV-curable, commercially available polymer with a refractive index (RI) matched to water. We show that cells can be directly grown on BIO-133 substrates without the need for surface passivation and use this capability to perform extended time-lapse volumetric imaging of cellular dynamics 1) at isotropic resolution using dual-view light-sheet microscopy, and 2) at super-resolution using instant structured illumination microscopy. BIO-133 also enables immobilization of 1) Drosophila tissue, allowing us to track membrane puncta in pioneer neurons, and 2) Caenorhabditis elegans, which allows us to image and inspect fine neural structure and to track pan-neuronal calcium activity over hundreds of volumes. Finally, BIO-133 is compatible with other microfluidic materials, enabling optical and chemical perturbation of immobilized samples, as we demonstrate by performing drug and optogenetic stimulation on cells and C. elegans.

中文翻译：

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与水相匹配的聚合物凝胶可实现荧光显微镜的多种应用

我们通过BIO-133的厚层（数百微米）展示了衍射极限和超分辨率成像，BIO-133是一种生物相容的，可紫外线固化的，商业上可获得的，折射率（RI）与水匹配的聚合物。我们表明细胞可以直接在BIO-133基底上生长而无需表面钝化，并使用此功能对细胞动力学进行扩展的延时体积成像； 1）使用双视角光片显微镜在各向同性分辨率下进行成像； 2使用即时结构化照明显微镜以超高分辨率拍摄）。BIO-133还可以固定1）果蝇组织，从而使我们能够追踪先驱神经元的膜点，以及2）秀丽隐杆线虫，这使我们能够成像和检查精细的神经结构，并跟踪数百个体积中的泛神经钙活动。最后，BIO-133与其他微流体材料兼容，能够对固定样品进行光学和化学扰动，正如我们通过对细胞和秀丽隐杆线虫进行药物和光遗传学刺激所证实的那样。