The insulin resistance state of pancreatic α-cells seems to be related to glucagon hypersecretion in type 2 diabetes. Treatment that can improve the insulin sensitivity of α-cells could control glucagon levels in patients with diabetes mellitus. The aim of this study was to investigate the preventive role of D-chiro-inositol (DCI), which has insulin receptor-sensitizer effects on insulin signaling pathways and glucagon secretion in pancreatic α-TC1 clone 6 cells. Cells were chronically treated with palmitate to induce insulin resistance in the presence/absence of DCI. DCI treatment improved the insulin signaling pathway and restored insulin-mediated glucagon suppression in α-TC1-6 cells exposed to palmitate. These results indicate that DCI treatment prevents the insulin resistance of α-TC1-6 cells chronically exposed to palmitate. Our data provide evidence that DCI could be useful to improve the insulin sensitivity of pancreatic α-cells in diabetes treatment.

中文翻译：

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D-手性肌醇对胰腺α细胞胰岛素信号传导和胰高血糖素分泌的直接影响

胰腺 α 细胞的胰岛素抵抗状态似乎与 2 型糖尿病患者的胰高血糖素分泌过多有关。可以改善α细胞胰岛素敏感性的治疗可以控制糖尿病患者的胰高血糖素水平。本研究的目的是研究 D-手性肌醇 (DCI) 的预防作用，D-手性肌醇对胰腺 α-TC1 克隆 6 细胞中的胰岛素信号通路和胰高血糖素分泌具有胰岛素受体敏化作用。在存在/不存在 DCI 的情况下，用棕榈酸酯长期处理细胞以诱导胰岛素抵抗。DCI 治疗改善了胰岛素信号通路并恢复了暴露于棕榈酸酯的 α-TC1-6 细胞中胰岛素介导的胰高血糖素抑制。这些结果表明 DCI 治疗可防止长期暴露于棕榈酸酯的 α-TC1-6 细胞的胰岛素抵抗。