ABSTRACT In this study, phylogenetic analysis of some Turkish Prunus armeniaca L. genotypes was conducted based on RAPD-PCR, ISSR-PCR, and chloroplast DNA (trnL-F) sequence analyses. Prunus armeniaca genotypes were obtained from Malatya province, and brought to the laboratory for genomic DNA isolation. Eleven RAPD and fifteen ISSR primers were used to determine molecular characterization of Prunus armeniaca genotypes. For the amplification of the trnL-F region, trne and trnf primers were used in Polymerase Chain Reaction (PCR). A total of 46 and 95 bands were obtained with RAPD and ISSR analysis, respectively. The trnL-F sequences varied from 398 to 403 nucleotides. Average nucleotide composition of trnL-F sequences was 31.0% T, 14.4% C, 37.4% A and 17.2% G. The genetic distance between the five apricot genotypes is 0.00. A maximum likelihood tree was generated to determine genetic relationships among Prunus armeniaca genotypes. Analysis of Prunus armeniaca genotypes based on RAPD, ISSR, and trnL-F sequences revealed that the rate of polymorphism obtained using RAPD and ISSR were higher than those obtained through the trnL-F sequences.

中文翻译：

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通过 RAPD、ISSR-PCR 和叶绿体 DNA (cpDNA) trnL-F 序列分析山梅（蔷薇科）基因型的分子遗传多样性

摘要 在本研究中，基于 RAPD-PCR、ISSR-PCR 和叶绿体 DNA (trnL-F) 序列分析，对一些土耳其李属亚美尼亚李基因型进行了系统发育分析。Prunus armeniaca 基因型从马拉蒂亚省获得，并带到实验室进行基因组 DNA 分离。使用 11 种 RAPD 和 15 种 ISSR 引物来确定 Prunus armeniaca 基因型的分子特征。为了扩增 trnL-F 区域，在聚合酶链反应 (PCR) 中使用了 trne 和 trnf 引物。RAPD 和 ISSR 分析分别获得了 46 个和 95 个条带。trnL-F 序列从 398 到 403 个核苷酸不等。trnL-F 序列的平均核苷酸组成为 31.0% T、14.4% C、37.4% A 和 17.2% G。五种杏基因型之间的遗传距离为 0.00。生成最大似然树以确定 Prunus armeniaca 基因型之间的遗传关系。基于 RAPD、ISSR 和 trnL-F 序列的山杏基因型分析表明，使用 RAPD 和 ISSR 获得的多态性率高于通过 trnL-F 序列获得的多态性。