Abstract

Estimates of Within-Subject and between subject biological variation for the white blood cell differential count (DC) have not been reported in South Asia. Therefore, we attempted to measure the short-term biological variation estimates for DC. The study was conducted on 28 healthy volunteers (15 males and 13 females). Blood from the volunteers was collected in the morning in K3-EDTA vials and analyzed in triplicate on the Sysmex XN-1000 analyzer, for six consecutive days. The Within subject, between subject and analytical coefficient of variation of the DC was calculated from the results by nested repeated measures ANOVA after outlier exclusion. The Reference change values (RCV) were also calculated. The within-subject variation for eosinophil Count and between subject variation for basophils in our study from South Asia was greater than the published European and American studies. Males and females showed similar biological variation for DC. The within-subject variation of other parameters (Neutrophils, Lymphocytes, Monocytes and Basophils) were similar or showed only mild differences to the published studies. The markedly different within-subject variation for Eosinophil counts suggest that the RCV for DC in South Asians need to be different from the published data in order to have clinical relevance. The Within-subject variation values of the other parameters seem transportable from the published European and American studies, but the small differences found mean that further regional estimates need to be reported for robust evidence of the same.

摘要

南亚尚未报告对白细胞分类计数 (DC) 的受试者内和受试者间生物学变异的估计。因此，我们试图测量 DC 的短期生物变异估计。该研究对 28 名健康志愿者（15 名男性和 13 名女性）进行。早上在 K3-EDTA 小瓶中收集志愿者的血液，并在 Sysmex XN-1000 分析仪上一式三份进行分析，连续六天。通过嵌套重复测量方差分析在异常值排除后从结果计算 DC 的内部受试者、受试者和分析变异系数。还计算了参考变化值 (RCV)。在我们来自南亚的研究中，嗜酸性粒细胞计数的受试者内变异和嗜碱性粒细胞受试者之间的变异大于已发表的欧洲和美国研究。雄性和雌性对 DC 表现出相似的生物学变异。其他参数（中性粒细胞、淋巴细胞、单核细胞和嗜碱性粒细胞）的受试者内变异与已发表的研究相似或仅表现出轻微差异。嗜酸性粒细胞计数显着不同的受试者内变异表明南亚人 DC 的 RCV 需要与已发表的数据不同才能具有临床相关性。其他参数的受试者内变异值似乎可以从已发表的欧洲和美国研究中转移，