ABSTRACT

1. Ovarian transcriptomic profiling between birds showing high egg number (HEN) and birds of low egg number (LEN) in Longyan Shan-ma ducks at 71 weeks of age was carried out using Illumina Hiseq 2500 technology.

2. A total of 343 differentially expressed genes (DEGs, 269 upregulated and 74 downregulated) were identified between HEN and LEN ovaries. These DEGs were enriched in 30 Gene Ontology terms. Pathway functional analysis found that the DEGs were enriched in 10 metabolic pathways (P < 0.05), one of which was regulation of the actin cytoskeleton pathway (Q < 0.05).

3. Three integrin family genes, ITGB2, ITGB5 and ITGA8 were differentially expressed in the RNA-seq and qPCR experiments.

4. The DEGs and signalling pathways identified in ovarian tissue in this study provide new insights into high egg production in Longyan Shan-ma duck.

摘要

1.使用Illumina Hiseq 2500技术对龙岩山麻鸭在71周龄时显示高蛋数（HEN）和低蛋数（LEN）的鸟类进行卵巢转录组谱分析。

2.在HEN和LEN卵巢之间共鉴定了343个差异表达基因（DEG，269个上调和74个下调）。这些DEG丰富了30种基因本体论术语。途径功能分析发现，DEGs富含10种代谢途径（P <0.05），其中之一是肌动蛋白细胞骨架途径的调节（Q <0.05）。

3.在RNA-seq和qPCR实验中差异表达了三个整合素家族基因ITGB2，ITGB5和ITGA8。

4.在这项研究中，在卵巢组织中鉴定出的DEG和信号传导途径为龙岩山麻鸭的高产卵提供了新的见解。