Abstract

This study evaluated the phytochemical characterization of Bixa orellana (BO extract) unsaponifiable extract and resulting fractions (F fraction - FF, Geranyl fraction – GF and R fraction- RF) obtained as by-products of an industrial process investigating in vitro antiproliferative activities in human tumoral cells. The main compounds identified by GC-MS for BO extract were Geranylgeraniol (61.51%); for FF: Geranylgeraniol (70.23%); for GF: Geranylgeraniol (78.92%) and for RF: β-cubebene (27.75%). Quantifications of geranylgeraniol by GC-FID presented the percentage content: BO 27.52%; FF 38.52%; GF 51.44% and RF 1.81%. BO extract showed a significant antiproliferative activity, with GI₅₀ up to 4 µg/mL. All fractions had a remarkably similar antiproliferative activity profile (GI₅₀ 27-47 µg/mL). Data reported herein showed an important cytostatic effect for BO extract, nevertheless this activity is not attributed exclusively to geranylgeraniol. In conclusion, this by-product becomes of great value, being a potential candidate for development of new anti-tumor ingredients.

摘要

本研究评估了Bixa orellana（BO 提取物）不皂化物提取物和作为工业过程副产品获得的馏分（F 馏分 - FF、香叶基馏分 - GF 和 R 馏分 - RF）的植物化学特性，该工业过程研究人类体外抗增殖活性肿瘤细胞。BO提取物GC-MS鉴定的主要化合物为香叶基香叶醇（61.51%）；对于 FF：香叶基香叶醇 (70.23%)；对于 GF：香叶基香叶醇 ( 78.92%) 和对于 RF：β- cubebene (27.75%)。GC-FID 对香叶基香叶醇的定量显示百分比含量：BO 27.52%；FF 38.52%；GF 51.44% 和 RF 1.81%。BO 提取物显示出显着的抗增殖活性，GI ₅₀高达 4 µg/mL。所有馏分都具有非常相似的抗增殖活性特征 (GI ₅₀ 27-47 µg/mL)。此处报告的数据显示 BO 提取物具有重要的细胞抑制作用，但是这种活性不仅仅归因于香叶基香叶醇。总之，这种副产品具有很高的价值，是开发新的抗肿瘤成分的潜在候选者。