Abstract

Complete mitochondrial genome of the Characiform fish Nematobrycon palmeri was characterized in the present study. The whole mitogenome was 17,340 bp in size and the proportion of coding sequences with a total length of 11,448 bp was 66.02%, which encodes 3805 amino acids. The base composition of the genome was 30.92% for A, 23.92% for C, 14.88% for G, and 30.28% for T. All protein-coding genes were started with ATG, CO1 and ATP8 ended by AGG, TAG respectively, whereas CO2, ATP6, ND4 ended by a single T, the other PCGs commonly ended by TAA. The length of 12S and 16S ribosomal RNA was 949 bp and 1675 bp, respectively. The control region (D-loop) ranging from 15,654 bp to 17,340 bp was 1687 bp in size. It showed negative GC skew value (–0.2329) and positive AT skewness (0.0105). Phylogenetic analysis showed that N. palmeri was most closely related to Gephyrocharax atracaudatus. The complete mitochondrial genome sequence would provide a new insight into taxonomic classification, and help to draw a more complete picture of species diversity within the Characidae.

摘要

Characiform鱼Nematobrycon palmeri的完整线粒体基因组在本研究中被表征。整个有丝分裂基因组的大小为17,340 bp，编码序列的总长度为11,448 bp的比例为66.02％，编码3805个氨基酸。基因组的基本组成是A的30.92％，C的23.92％，G的14.88％，T的30.28％。所有蛋白质编码基因均以ATG，CO1和ATP8开头，分别以AGG，TAG结束，而CO2 ，ATP6，ND4以单个T结尾，其他PCG通常以TAA结尾。12S和16S核糖体RNA的长度分别为949 bp和1675 bp。从15654 bp到17340 bp的控制区（D环）大小为1687 bp。它显示出负的GC偏斜值（–0.2329）和正的AT偏斜（0.0105）。系统发育分析表明，N. palmeri与猪气单胞菌（Gephyrocharax atracaudatus）。完整的线粒体基因组序列将为分类学分类提供新的见解，并有助于绘制出Characidae内物种多样性的更完整图景。