Abstract

This study investigated the effects of hydrocarbon-degrading bacteria and organic matter on a crude oil-polluted soil by Cordia myxa. The treatments consisted of crude oil at two levels (3 and 6% w/w), municipal waste compost at two levels (5 and 10% v/v), and two different bacterial strains (Pseudomonas sp.141 and Pseudomonas sp. 27ps). At the end of the growth period, the plants were harvested and prepared for the laboratory analyses. The greatest population of oil degrading-bacteria (4.6 × 10⁶ CFU/g soil) was observed in the treatment containing 10% compost, 6% crude oil, and Pseudomonas sp.141. The highest crude oil degradation (76.49%) was recorded in the soil polluted with 6% crude oil, amended with 10% compost, and inoculated with Pseudomonas sp.141. The investigation on the degradation of the chains of C10–C35 compounds indicated that, in various treatments, the most abundant compound was among those with fewer carbon atoms (C12–C25), so the application of organic matter boosted the degradation of crude oil. In conclusion, C. myxa seedlings has an acceptable efficiency in the remediation of the oil-contaminated soil affected by biological factors (compost and Pseudomonas bacteria), which is because of their high tolerance to the pollution and their ability to penetrate deeper soil layers.

抽象的

本研究探讨了Cordia myxa对降解原油的土壤中碳氢化合物降解细菌和有机物的影响。处理包括两个级别的原油（3和6％w / w），两个级别的城市垃圾堆肥（5％和10％v / v）以及两种不同的细菌菌株（假单胞菌sp.141和假单胞菌sp。27ps）。 ）。在生长期结束时，收获植物并准备进行实验室分析。在含10％堆肥，6％原油和假单胞菌的处理中，观察到最大的石油降解细菌种群（4.6×10 ⁶ CFU / g土壤）141。在受6％原油污染，经10％堆肥修正并接种假单胞菌（Pseudomonas sp.141 ）的土壤中，原油降解最高（76.49％）。对C10–C35化合物链降解的研究表明，在各种处理中，碳原子数较少的化合物（C12–C25）中含量最高的化合物，因此有机物的应用促进了原油的降解。总之，粘液梭菌幼苗在受生物因素（堆肥和假单胞菌细菌）影响的被油污染的土壤的修复中具有可接受的效率，这是因为它们对污染的高度耐受性以及它们能够穿透更深的土壤层的能力。