This experiment was performed to evaluate the possible embryotoxic and teratogenic effects of doxycycline during rat development. Twenty‐one female rats were used and distributed into three groups equally (seven animals/group). The low dose group received doxycycline at a dose of 5 mg/kg bw/day orally from the 6th to 14th day of gestation. The high dose group received 10 mg/kg bw/day orally for the same period, the Control group received 1 mL distilled water orally for the same period. The dams were dissected on the 20th day of gestation and their fetuses were subjected to morphological, skeletal, and histological examination. Moreover, DNA damage analysis of liver cells of pregnant rats and their fetuses or fetal skull was assessed by Comet assay. The obtained results showed a significant decrease in fetal body weight, several morphological anomalies, and severe lack of ossification on the skull bones, phalanges, and sternum bone as well as shortness in the ulna and radius bones. Histological studies of pregnant rats revealed congestion and dilatation of the central vein of the liver lobules and fatty degeneration of the hepatocytes. In addition, 20 day‐fetuses showed a marked increase of necrotic hepatocytes associated with an increased average of megakaryocytes and periportal leukocytic infiltration. Moreover, doxycycline induced a significant increase in the percentage of DNA damage and tail length of examined samples. Conclusively, doxycycline caused certain fetal abnormalities, so it is advisable to avoid using this drug during pregnancy.

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强力霉素对器官发生过程中大鼠胚胎发育的影响

进行该实验以评估强力霉素在大鼠发育过程中可能产生的胚胎毒性和致畸作用。使用了21只雌性大鼠并将其平均分为三组（每组七只动物）。低剂量组从妊娠第6天到第14天口服5毫克/千克体重/天的强力霉素。高剂量组在同一时期内口服10 mg / kg bw /天，对照组在同一时期内口服1 mL蒸馏水。在妊娠的第20天解剖水坝，并对胎儿进行形态，骨骼和组织学检查。此外，通过Comet试验评估了妊娠大鼠及其胎儿或胎儿头骨的肝细胞的DNA损伤分析。获得的结果显示胎儿体重显着降低，几个形态异常，头骨，指骨和胸骨严重骨化，尺骨和radius骨骨短。怀孕大鼠的组织学研究表明，肝小叶中心静脉充血和扩张，肝细胞脂肪变性。另外，20天胎儿显示坏死性肝细胞显着增加，与巨核细胞平均增加和门静脉周围白细胞浸润有关。此外，强力霉素可导致DNA损伤百分比和被检样品尾巴长度显着增加。总之，强力霉素会导致某些胎儿异常，因此建议在怀孕期间避免使用这种药物。和胸骨，以及尺骨和radius骨的短小。怀孕大鼠的组织学研究表明，肝小叶中心静脉充血和扩张，肝细胞脂肪变性。另外，20天胎儿显示坏死性肝细胞显着增加，与巨核细胞平均增加和门静脉周围白细胞浸润有关。此外，强力霉素可导致DNA损伤百分比和被检样品尾巴长度显着增加。总之，强力霉素会导致某些胎儿异常，因此建议在怀孕期间避免使用这种药物。和胸骨，以及尺骨和radius骨的短小。怀孕大鼠的组织学研究表明，肝小叶中心静脉充血和扩张，肝细胞脂肪变性。另外，20天胎儿显示坏死性肝细胞显着增加，与巨核细胞平均增加和门静脉周围白细胞浸润有关。此外，强力霉素可导致DNA损伤百分比和被检样品尾巴长度显着增加。总之，强力霉素会导致某些胎儿异常，因此建议在怀孕期间避免使用这种药物。20天胎儿显示坏死性肝细胞显着增加，伴随着巨核细胞平均增加和门静脉周围白细胞浸润。此外，强力霉素可导致DNA损伤百分比和被检样品尾巴长度显着增加。总之，强力霉素会导致某些胎儿异常，因此建议在怀孕期间避免使用这种药物。20天胎儿显示坏死性肝细胞显着增加，与巨核细胞平均增加和门静脉周围白细胞浸润有关。此外，强力霉素可导致DNA损伤百分比和被检样品尾巴长度显着增加。总之，强力霉素会导致某些胎儿异常，因此建议在怀孕期间避免使用这种药物。