The purpose of this study was to investigate the impact of eggshell calcium (Biomin H^® dietary supplement) and its combinations with alfacalcidol (1α‐hydroxyvitamin D₃) and menaquinone‐7 (vitamin K₂) on ovariectomy‐induced bone loss in rats. Adult female rats (n = 48) were divided into 6 groups of 8 individuals each: sham‐operated rats (SHAM); ovariectomized (OVX) rats untreated; OVX rats treated with Biomin H^® (BIO); OVX rats simultaneously receiving Biomin H^®, vitamin D₃ (BIO + D₃); OVX rats simultaneously treated with Biomin H^®, vitamin K₂ (BIO + K₂) and OVX rats treated with Biomin H^®, vitamin D₃, vitamin K₂ (BIO + D₃ + K₂) during 8 weeks. Biochemical parameters, bone mineral density (BMD), bone mineral content (BMC) and femoral bone microstructure were determined. Plasma calcium and phosphate were increased in BIO + D₃ and BIO + D₃ + K₂ groups as compared to OVX. Alkaline phosphatase was elevated in OVX, BIO versus SHAM, BIO + D₃ + K2 groups. When compared to OVX group, decreased urine deoxypyridinoline was observed in all treated groups and femoral BMD, BMC were higher in BIO, BIO + D₃, BIO + D₃ + K₂ groups. The BIO + K₂ rats had similar densitometrical values than OVX individuals. Microcomputed tomography revealed increased trabecular relative bone volume (due to an increase in trabecular number) in BIO + D₃, BIO + D₃ + K₂ as compared to OVX. The higher relative bone volume in BIO + D₃, BIO + D₃ + K₂ groups was also accompanied by an increase in bone surface. In the cortical bone, an enhanced periosteal bone apposition was identified in BIO, BIO + D₃, BIO + K₂, BIO + D₃ + K₂ groups. The rats from BIO + D₃ + K₂ group had a higher area of primary osteon's vascular canals. In BIO + D₃, BIO + K₂, BIO + D₃ + K₂ groups, an increased area of secondary osteons was determined in comparison with OVX. Our results indicate the beneficial effect of triple application of Biomin H^®, vitamin D₃, vitamin K₂, as well as simultaneous administration of Biomin H^®, vitamin D₃ on the inhibition of ovariectomy‐induced bone loss in a rat model of osteoporosis.

中文翻译：

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蛋壳钙 (Biomin H®) 及其与阿法骨化醇（1α-羟基维生素 D3）和甲基萘醌-7（维生素 K2）的组合对骨质疏松症大鼠模型中卵巢切除引起的骨丢失的影响


本研究的目的是研究蛋壳钙（Biomin H ^®膳食补充剂）及其与阿法骨化醇（1α-羟基维生素 D ₃ ）和甲萘醌-7（维生素 K ₂ ）的组合对卵巢切除引起的大鼠骨质流失的影响。成年雌性大鼠（ n = 48）分为 6 组，每组 8 只：假手术大鼠（SHAM）；未经治疗的卵巢切除（OVX）大鼠；用 Biomin H ^® (BIO) 处理的 OVX 大鼠； OVX大鼠同时接受Biomin H ^® 、维生素D ₃ (BIO + D ₃ )； OVX 大鼠同时接受 Biomin H ^® 、维生素 K ₂ (BIO + K ₂ ) 治疗，OVX 大鼠同时接受 Biomin H ^® 、维生素 D ₃ 、维生素 K ₂ (BIO + D ₃ + K ₂ ) 治疗，为期 8 周。测定生化参数、骨矿物质密度（BMD）、骨矿物质含量（BMC）和股骨微结构。与OVX相比，BIO + D ₃和BIO + D ₃ + K ₂组的血浆钙和磷酸盐增加。与SHAM、BIO + D ₃ + K2 组相比，OVX、BIO 组的碱性磷酸酶升高。与OVX组相比，所有治疗组的尿脱氧吡啶啉均减少，BIO、BIO + D ₃ 、BIO + D ₃ + K ₂组的股骨BMD、BMC较高。 BIO + K ₂大鼠与 OVX 个体具有相似的光密度值。 微计算机断层扫描显示，与 OVX 相比，BIO + D ₃ 、BIO + D ₃ + K ₂中的小梁相对骨体积增加（由于小梁数量增加）。 BIO+D ₃ 、BIO+D ₃ +K ₂组相对骨体积较高，同时骨表面也增加。在皮质骨中，BIO、BIO + D ₃ 、BIO + K ₂ 、BIO + D ₃ + K ₂组中骨膜骨附着增强。 BIO+D ₃ +K ₂组大鼠原代骨血管管面积较高。在BIO+D ₃ 、BIO+K ₂ 、BIO+D ₃ +K ₂组中，与OVX相比，确定次级骨面积增加。我们的结果表明，三联应用 Biomin H ^® 、维生素 D ₃ 、维生素 K ₂以及同时给药 Biomin H ^® 、维生素 D ₃对抑制骨质疏松大鼠模型中卵巢切除引起的骨丢失具有有益作用。