Abstract The enzymatic polymerization of rutin has been proved as an efficient method for obtaining products with enhanced properties, but toxic organic co-solvents are often involved in this process. In this work, the effect of temperature and enzyme activity were initially studied on 24 h batch oligomerization reactions in organic-free reaction media. The optimal conditions, 1000 U/L and 25 °C, were chosen to operate an ultrafiltration enzymatic membrane reactor (UF-EMR) during three consecutive oligorutin synthesis cycles of 6 and 24 h, with the objective of reusing the initial laccase activity. The use of this technology led to the design of a biocompatible and sustainable process with complete rutin conversion and remarkable laccase stability in at least three consecutive 24 h cycles with no further enzyme addition. Despite an approximately 80% lower ferric reducing antioxidant power (FRAP), oligorutin displayed up to 1620-fold aqueous solubility and 24-fold iron chelating activity. Additionally, tests involving DNA degradation inhibition in exposure to biologically related radicals showed a better performance of oligorutin when compared to rutin.

中文翻译：

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使用酶膜反应器绿色可持续合成寡糖苷：工艺优化

摘要 芦丁的酶促聚合已被证明是一种获得性能增强产品的有效方法，但该过程中经常涉及有毒的有机助溶剂。在这项工作中，最初研究了温度和酶活性对无有机反应介质中 24 小时分批低聚反应的影响。选择最佳条件 1000 U/L 和 25°C，在 6 小时和 24 小时的三个连续寡糖苷合成循环期间操作超滤酶膜反应器（UF-EMR），目的是重复利用初始漆酶活性。这项技术的使用导致设计了一种生物相容性和可持续的工艺，在至少三个连续的 24 小时循环中完全转化芦丁并具有显着的漆酶稳定性，无需进一步添加酶。尽管铁还原抗氧化能力 (FRAP) 降低了约 80%，但寡糖苷的水溶性和铁螯合活性高达 1620 倍和 24 倍。此外，涉及在暴露于生物相关自由基时抑制 DNA 降解的测试表明，与芦丁相比，oligorutin 具有更好的性能。