Sulfur-oxidizing bacteria that are halophilic and acidophilic have gained interest because of their potential use in bioleaching operations in salt-containing environments. Acidithiobacillus sp. strain SH, which was previously identified as Acidithiobacillus thiooxidans based on its 16S rRNA gene sequence, is a chemolithoautotrophic marine bacterium exhibiting sodium chloride-stimulated thiosulfate-oxidizing activities. A novel thiosulfate:quinone oxidoreductase from strain SH (SH-TQO) has been purified from its solubilized membrane fraction. The gene for SH-TQO was determined from the draft genome sequence of the strain SH. Amino acid sequences of peptides generated by the in-gel trypsin digestion of SH-TQO were found in a protein encoded by locus tag B1757_09800 of the genome of the strain SH. The gene encoded 444 amino acids with a signal peptide of 29 amino acids and was annotated to encode a porin. The gene was located in a unique genomic region, not found in A. thiooxidans strains, suggesting that the strain SH acquired this region through a horizontal gene transfer. A protein–protein basic local alignment search revealed that sulfur-oxidizing bacteria, such as Acidithiobacillus species have proteins homologous to SH-TQO, though the degree of homologies was relatively low. The protein, DoxXA, which is homologous to TQO from Acidianus amvibalens, was also found in the genomic region.

嗜盐和嗜酸性的硫氧化细菌由于其在含盐环境中生物浸提操作中的潜在用途而引起了人们的兴趣。嗜酸菌。SH菌株，以前被鉴定为酸性硫氧化杆菌基于其16S rRNA基因序列，是一种化学自养型海洋细菌，具有氯化钠刺激的硫代硫酸盐氧化活性。从菌株SH（SH-TQO）获得的新型硫代硫酸盐：醌氧化还原酶已从其溶解的膜级分中纯化出来。SH-TQO的基因是从SH菌株的基因组草图序列中确定的。在SH菌株基因组标签B1757_09800编码的蛋白质中发现了SH-TQO的凝胶内胰蛋白酶消化产生的肽的氨基酸序列。该基因编码444个氨基酸，带有29个氨基酸的信号肽，并注释为编码孔蛋白。该基因位于一个独特的基因组区域，而在硫氧化农杆菌中找不到菌株，表明SH菌株通过水平基因转移获得了该区域。蛋白质-蛋白质基本的局部比对研究表明，硫氧化细菌（如嗜酸性硫杆菌属）具有与SH-TQO同源的蛋白质，尽管同源性相对较低。在基因组区域中也发现了与来自酸性嗜酸杆菌的TQO同源的蛋白质DoxXA 。