Anthocyanins are a group of secondary metabolites that protect plants from biotic and abiotic stresses. The research on anthocyanins has been well-received due to their colorfulness and human health benefits. In this study, we used the photosensitive eggplant cultivar 'Lanshan Hexian' as the research material and reported the functional characterization of SmMYB86, a negative regulator involved in the anthocyanin biosynthesis in eggplant. Our results suggested that SmMYB86 was a nuclear protein that was particularly expressed in leaves, stems, and peels. Overexpression of SmMYB86 in eggplant indicated that the accumulation of anthocyanins was reduced. Silencing of SmMYB86 in eggplant fruit peel significantly increased the anthocyanin content and expression levels of SmCHS, SmF3H, and SmANS. Yeast one-hybrid and dual-luciferase assays showed that SmMYB86 could directly bind to the promoters of SmCHS, SmF3H, and SmANS and suppress their activities. SmTTG1 binded to the promoter of SmCHS and promoted its activating. SmMYB86 interacted with SmTTG1 and inhibited its promotive role in SmCHS expression. This study provides some insights into the regulatory roles of SmMYB86 on key structural genes in the anthocyanin synthesis pathway in eggplant.

中文翻译：

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SmMYB86 的功能表征，茄子花青素生物合成的负调节因子 (Solanum melongena L.)

花青素是一组次级代谢产物，可保护植物免受生物和非生物胁迫。花青素的研究因其色彩丰富和对人体健康有益而广受好评。在本研究中，我们以光敏茄子品种'蓝山和弦'为研究材料，报道了茄子花青素生物合成中的负调控因子SmMYB86的功能表征。我们的结果表明 SmMYB86 是一种核蛋白，在叶、茎和果皮中特别表达。SmMYB86在茄子中的过表达表明花青素的积累减少。茄子果皮中 SmMYB86 的沉默显着增加了 SmCHS、SmF3H 和 SmANS 的花青素含量和表达水平。酵母单杂交和双荧光素酶测定表明 SmMYB86 可以直接与 SmCHS、SmF3H 和 SmANS 的启动子结合并抑制它们的活性。SmTTG1 与 SmCHS 的启动子结合并促进其激活。SmMYB86 与 SmTTG1 相互作用并抑制其在 SmCHS 表达中的促进作用。本研究为 SmMYB86 对茄子花青素合成途径关键结构基因的调控作用提供了一些见解。