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GmbZIP45 binds to H-box cis-element in vitro and overexpression in soybean protoplasts induces the expression of CHS8 gene
Physiological and Molecular Plant Pathology ( IF 2.8 ) Pub Date : 2020-12-01 , DOI: 10.1016/j.pmpp.2020.101556
Amanda Bonoto Gonçalves , Patrícia Pereira Fontes , Silvana Pinheiro Dadalto , Gilza Barcelos de Souza , Francismar Corrêa Marcelino-Guimarães , Murilo Siqueira Alves , Luciano Gomes Fietto

Abstract Previous work has shown that GmbZIP45 expression is induced upon Phakopsora pachyrhizi infection. In the present work, it is shown P. pachyrhizi infection induces the expression of some phenylpropanoid biosynthesis genes. GmbZIP45 binds to the H-box, cis-element present on promoters of such genes. Interestingly, overexpression of GmbZIP45 activates the transcription of CHS8 but does not induce the expression of the other genes analyzed. Taken together, our data suggest interaction with H-box in a cooperative way along with other transcription factors can be a potential mechanism of regulation of targets genes by GmbZIP45.

中文翻译:

GmbZIP45 在体外结合 H-box 顺式元件,在大豆原生质体中过表达诱导 CHS8 基因的表达

摘要 先前的工作表明 GmbZIP45 表达在 Phakopsora pachyrhizi 感染后被诱导。在目前的工作中,表明 P. pachyrhizi 感染诱导了一些苯丙烷生物合成基因的表达。GmbZIP45 与 H 盒、此类基因启动子上存在的顺式元件结合。有趣的是,GmbZIP45 的过表达会激活 CHS8 的转录,但不会诱导其他分析基因的表达。综上所述,我们的数据表明,以合作方式与 H-box 以及其他转录因子的相互作用可能是 GmbZIP45 调控靶基因的潜在机制。
更新日期:2020-12-01
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