The presence of biosurfactants produced by a Bacillus strain in corn steep liquor (CSL), a wastewater stream of the corn milling process, has been recently discovered. However, the species responsible for their production has not been identified at the moment. Therefore, in this work, the Bacillus strain isolated from CSL, with capacity to produce biosurfactants, was subjected to amplification and sequence analysis of the 16S rRNA, being identified as Aneurinibacillus aneurinilyticus. This strain has been proved to be endospore forming and thermophile, what would explain its presence in the commercial CSL. It was observed that the strain under evaluation has the ability to produce both cell-bound and extracellular biosurfactant extracts, which were characterized in this work. The electrospray ionization mass spectrometry (ESI) analysis of the biosurfactant extracts revealed that the extracellular biosurfactant produced by Aneurinibacillus aneurinilyticus is composed by a mixture of lipopeptides, containing C16 and C18 fatty acids and amino acids, including valine, phenylalanine, proline, cysteine, histidine, aspartic acid/asparagine, alanine, glycine, leucine/isoleucine, with biomarkers between 1025−458 m/z. Conversely, the cell-bound biosurfactant extract produced by Aneurinibacillus aneurinilyticus was composed by the cyclic decapeptide gramicidin S, with a characteristic peak at 571 m/z, and lipopeptides with characteristic peaks between 1034−705 m/z, containing alanine, glycine, cysteine, serine, proline, aspartic acid/asparagine, similarly to the amino acid sequence of the extracellular biosurfactant extract.

最近发现了芽孢杆菌属菌株在玉米浸泡液（CSL）中产生的生物表面活性剂（玉米研磨过程的废水）。但是，目前尚未确定负责其生产的物种。因此，在这项工作中，对从CSL分离的具有生产生物表面活性剂能力的芽孢杆菌菌株进行了16S rRNA的扩增和序列分析，该菌株被鉴定为解毒性阿氏杆菌。该菌株已被证明可形成内生孢子并具有嗜热性，这可以解释其在商业CSL中的存在。观察到，被评估的菌株具有产生细胞结合的和细胞外的生物表面活性剂提取物的能力，这是这项工作的特征。对生物表面活性剂提取物的电喷雾电离质谱（ESI）分析表明，由解热性动脉瘤杆菌产生的细胞外生物表面活性剂由脂肽的混合物组成，脂肽含有C16和C18脂肪酸和氨基酸，包括缬氨酸，苯丙氨酸，脯氨酸，半胱氨酸，组氨酸，天冬氨酸/天冬酰胺，丙氨酸，甘氨酸，亮氨酸/异亮氨酸，生物标记在1025-458 m / z之间。相反，解毒性动脉瘤杆菌生产的细胞结合的生物表面活性剂提取物由环状十肽短杆菌肽S组成，其特征峰在571 m / z，脂肽的特征峰在1034-705 m / z，包含丙氨酸，甘氨酸，半胱氨酸。 ，丝氨酸，脯氨酸，天冬氨酸/天冬酰胺，类似于细胞外生物表面活性剂提取物的氨基酸序列。