The endophytic bacteria were isolated from coffee roots and seeds in Vietnam and identified with 16S rDNA sequencing as belonging to the Actinobacteria, Firmicutes and Proteobacteria phyla with the Nocardia, Bacillus and Burkholderia as dominant genera, respectively. Out of the thirty genera recovered from Coffea canephora and Coffea liberica, twelve were reported for the first time in endophytic association with coffee including members of the genera Brachybacterium, Caballeronia, Kitasatospora, Lechevalieria, Leifsonia, Luteibacter, Lysinibacillus, Mycolicibacterium, Nakamurella, Paracoccus, Sinomonas and Sphingobium. A total of eighty bacterial endophytes were characterized in vitro for several plant growth promoting and biocontrol traits including: the phosphate solubilization, the indolic compounds, siderophores, HCN, esterase, lipase, gelatinase and chitinase production. A subset of fifty selected bacteria were tested for their potential as biocontrol agents with in vitro confrontations with the fungal pathogen Fusarium oxysporum as well as the coffee parasitic nematodes Radopholus duriophilus and Pratylenchus coffeae. The three most efficient isolates on F. oxysporum belonging to the Bacillus, Burkholderia, and Streptomyces genera displayed a growth inhibition rate higher than 40%. Finally, five isolates from the Bacillus genus were able to lead to 100% of mortality in 24 h on both R. duriophilus and P. coffeae.

从越南的咖啡根和种子中分离出内生细菌，并通过16S rDNA测序鉴定为内生细菌，分别属于放线菌，硬毛菌和变形杆菌，以诺卡氏菌，芽孢杆菌和伯克霍尔德菌为优势属。在从卡菲咖啡和自由咖啡中回收的三十个属中，首次报道了十二种与咖啡的内生结合，包括短枝杆菌属，Caballeronia，Kitasatospora，Lechevalieria，Leifsonia，Luteibacter，Lysinibacillus，Mycolicibacterium，Nakamurella，Paracoccus，中华和鞘氨醇。总共对八十种细菌内生菌进行了体外表征，鉴定了几种促进植物生长和生物防治的特性，包括：磷酸盐增溶，吲哚化合物，铁载体，HCN，酯酶，脂肪酶，明胶酶和几丁质酶的生产。测试了50种选定细菌的亚组作为生物防治剂的潜力，在体外与真菌病原镰刀菌，咖啡寄生线虫Radopholus duriophilus和Pratylenchus coffeae对峙。在三种最有效的分离尖孢镰刀菌属于芽孢杆菌，鼻疽，和链霉菌属显示出高于40％的生长抑制率。最后，来自芽孢杆菌属的五种分离物能够在24小时内导致杜氏嗜酸杆菌和咖啡杆菌的死亡率达到100％。