Polysaccharides from P. eryngii mushroom were selectively extracted using low-cost technologies (water at different conditions of temperature and pressure). Mannogalactan was the main polysaccharide in cold-water extracted fraction (CWEF), while a linear (1→6)-β-d-glucan was the main polymer in hot-water extracted fraction (HWEF). Autoclave-extracted fraction (AEF) contained a mixture of at least four different α- and β-glucans. The report of linear (1→6)-β-glucan and linear (1→3)-β-glucan is a new finding for P. eryngii fruiting bodies. The immunostimulatory properties of the fractions on THP-1 macrophages were studied. All fractions at 50, 250 and 500 μg/mL were not cytotoxic and produced different stimulus on NO, IL-1β and IL-10 secretion by the cells. Thus, our results showed that it is possible to concentrate different P. eryngii polysaccharides in selected fractions using a simple and low-cost procedure. Since biological effects depends on the polysaccharide structure, this technique allows the obtainment of fractions with distinct immunomodulatory activities.

使用低成本技术（在不同温度和压力条件下的水）选择性提取杏鲍菇蘑菇中的多糖。甘露半乳聚糖是冷水提取级分（CWEF）中的主要多糖，而线性（1→6）-β- d-葡聚糖是热水提取级分（HWEF）中的主要聚合物。高压釜提取的馏分（AEF）包含至少四种不同的α-和β-葡聚糖的混合物。线性（1→6）-β-葡聚糖和线性（1→3）-β-葡聚糖的报道是对杏鲍菇的新发现。子实体。研究了这些组分对THP-1巨噬细胞的免疫刺激特性。50、250和500μg/ mL的所有组分均无细胞毒性，并且对细胞分泌的NO，IL-1β和IL-10产生不同的刺激作用。因此，我们的结果表明，可以使用简单且低成本的方法将不同的杏鲍菇多糖浓缩成选定的级分。由于生物学作用取决于多糖结构，因此该技术可实现具有独特免疫调节活性的级分。