The microbial community structure in the throat and its shift after laryngectomy in laryngeal squamous cell carcinoma (LSCC) patients were investigated. Thirty swab samples taken prior to laryngectomy (SLC), 18 samples 1 week after laryngectomy (SLCA1w), and 30 samples 24 weeks after laryngectomy (SLCA24w) from 30 LSCC patients were examined. Microbial diversity was profiled through sequencing the V3-V4 variable region of the 16S rRNA gene. Quantitative real-time PCR (qPCR) was used to validate the 16S rRNA sequence data for the V3-V4 region. The community structure and function of throat microbiota were assessed by PICRUSt (phylogenetic investigation of communities by reconstruction of unobserved states) analysis. Both alpha and beta diversity results showed significant differences in the throat microbiota of LSCC patients before and after laryngectomy (P < 0.05). The drinking index of the SLC group was positively associated with the genus abundance of Prevotella (P < 0.05). The SLCA1w group had lower abundances of Fusobacterium, Leptotrichia, Lachnoanaerobaculum, and Veillonella than the SLC group (P < 0.05). The SLCA24w group had higher abundances of Streptococcus and Leptotrichia as well as lower abundances of Fusobacterium and Alloprevotella than the SLC group (P < 0.05). The throat microbiomes of the SLC group could be implicated in human cancer signaling pathways, as evidenced by PICRUSt analysis (P < 0.05). Our study clarifies alterations in throat microbial community structure and function in LSCC patients during the perioperative period and postoperative recovery period.

中文翻译：

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喉癌患者术后喉咙微生物群落结构和功能变化

研究了喉鳞状细胞癌（LSCC）患者喉咙中的微生物群落结构及其在喉切除后的移位。检查了30例LSCC患者在喉切除术（SLC）之前采集的30个拭子样本，喉切除术1周后（SLCA1w）的18个样本和喉切除术后24周（SLCA24w）的30个样本。通过对16S rRNA基因的V3-V4可变区进行测序，可对微生物多样性进行分析。实时定量PCR（qPCR）用于验证V3-V4区的16S rRNA序列数据。通过PICRUSt（通过重建未观察到的状态对群落进行系统发育研究）分析评估了喉咙微生物群的群落结构和功能。P <0.05）。SLC组的饮酒指数与普氏杆菌属的丰富度呈正相关（P <0.05）。该SLCA1w组具有较低的丰度梭杆菌，纤毛，Lachnoanaerobaculum和韦荣球菌比SLC组（P <0.05）。该SLCA24w组具有更高的丰度链球菌和纤毛以及低丰度梭杆菌和Alloprevotella比SLC组（P<0.05）。PICRUSt分析证明，SLC组的喉咙微生物群可能与人类癌症的信号通路有关（P <0.05）。我们的研究阐明了LSCC患者围手术期和术后恢复期喉咙微生物群落结构和功能的变化。