Optical coherence tomography microscopy in experimental traumatic brain injury,Microscopy Research and Technique

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Optical coherence tomography microscopy in experimental traumatic brain injury
Microscopy Research and Technique ( IF 2.0 ) Pub Date : 2020-10-03 , DOI: 10.1002/jemt.23599
Eugen Osiac _{1,

2} , Smaranda Ioana Mitran _{1,

3} , Cătălin Nicolae Manea _{1,

4} , Alexandru Cojocaru ₃ , Gabriela-Camelia Rosu ₅ , Mariana Osiac ₆ , Daniel Nicolae Pirici ₅ , Adrian Tudor Bălșeanu _{1,

3} , Bogdan Cătălin _{1,

3}

Affiliation

Worldwide elderly traumatic brain injury (TBI) patients tend to become an increasing burden to the society. Thus, a faster and less expensive way of evaluating TBI victims is needed. In the present study we investigated if optical coherence tomography (OCT) could be used as such a method. By using an animal model, we established if OCT can detect cortical changes in the acute phase of a penetrating TBI, in young (5–7 months) and old (20–22 months) rats. Due to the long‐term evolution of TBI's, we wanted to investigate to what extent OCT could detect changes within the cortex in the chronic phase. Adult (7–12 months) male rats were used. Surprisingly, OCT imaging of the normal hemisphere was able to discriminate age‐related differences in the mean gray values (MGV) of recorded pixels (p = .032). Furthermore, in the acute phase of TBI, OCT images recorded at 24 hr after the injury showed differences between the apparent damaged area of young and aged animals. Changes of MGV and skewness were only recorded 48 hr after injury. Monitoring the chronical evolution of the TBI with OCT revealed changes over time exceeding the normal range recorded for MGV, skewness and kurtosis, 14 and 21 days after TBI. Although in the present study we still used an extremely invasive approach, as technology improves, less invasive and non‐harmful ways of recording OCT may allow for an objective way to detect changes within the brain structure after brain injuries.

中文翻译：

光学相干断层扫描显微镜在实验性脑外伤中的应用

全球范围内的老年创伤性脑损伤（TBI）患者往往会成为日益增加的社会负担。因此，需要一种更快、更便宜的方法来评估 TBI 受害者。在本研究中，我们研究了光学相干断层扫描（OCT）是否可以用作这种方法。通过使用动物模型，我们确定 OCT 是否可以检测年轻（5-7 个月）和老年（20-22 个月）大鼠穿透性 TBI 急性期的皮质变化。由于 TBI 的长期演变，我们想要研究 OCT 在多大程度上可以检测到慢性期皮质内的变化。使用成年（7-12 个月）雄性大鼠。令人惊讶的是，正常半球的 OCT 成像能够区分记录像素的平均灰度值 (MGV) 与年龄相关的差异 ( p = .032)。此外，在 TBI 的急性期，受伤后 24 小时记录的 OCT 图像显示年轻和老年动物的明显受损区域之间存在差异。仅在受伤后 48 小时记录 MGV 和偏度的变化。使用 OCT 监测 TBI 的长期演变显示，TBI 后 14 天和 21 天的 MGV、偏度和峰度随时间的变化超出了记录的正常范围。尽管在本研究中我们仍然使用一种极具侵入性的方法，但随着技术的进步，记录 OCT 的侵入性较小且无害的方法可能会提供一种客观的方法来检测脑损伤后大脑结构的变化。

更新日期：2020-10-03

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