The role of biochar has been identified in soil organic carbon (SOC) mineralization, but the role of dissolved organic carbon (DOC) derived from biochar water extract (BE) is not well recognized. Therefore, rotary tillage (RT) and no-tillage (NT) with two moisturizers treatments; BE and distilled water (WA) were used to study the influence of DOC (through BE) on SOC mineralization and C fractions. Four soil sampling depths (0-10, 10-20, 20-30, 30-50 cm) were used for laboratory incubation. The results showed a significant increase in cumulative CO₂-C emissions in upper soil that decreased with increase in soil depth. BE increased cumulative CO₂-C emissions about 50% and 46% (0-10 cm), 45% and 26% (10-20 cm), 42% and 55% (20-30 cm), 16% and 43% (30-50 cm) than WA in NT and RT, respectively. Moreover, NT had 13% more cumulative CO₂-C emissions than RT at 0-10 cm, but RT had 10% and 38% higher cumulative CO₂-C emissions than NT at 10-20 and 20-30 cm soil depths, respectively. BE increased microbial quotient (Mq) almost 47%, 70%, 24% and 167% at 0-10, 10-20, 20-30 and 30-50 cm soil depths, respectively. Increase in Mq significantly decreased SOC and some carbon fractions. Notably, BE did not modify DOC in whole soil profile under both tillage systems. BE had significant higher MBC at 30-50 cm soil depth than WA in both tillage systems. RT had significant higher (4%) MBC than NT at 20-30 cm soil depth. Overall, addition of DOC from BE in soil enhances C mineralization by modifying Mq.

虽然已经确定了生物炭在土壤有机碳（SOC）矿化中的作用，但是尚未充分认识到源自生物炭水提取物（BE）的溶解有机碳（DOC）的作用。因此，旋耕（RT）和免耕（NT）采用两种保湿剂处理；用BE和蒸馏水（WA）研究DOC（通过BE）对SOC矿化和碳分数的影响。四种土壤采样深度（0-10、10-20、20-30、30-50 cm）用于实验室培养。结果表明，上部土壤中累积的CO ₂ -C排放量显着增加，随土壤深度的增加而减少。被增加的累积CO ₂-C排放量约为50％和46％（0-10厘米），45％和26％（10-20厘米），42％和55％（20-30厘米），16％和43％（30-50厘米） ）分别比NT和RT中的WA高。此外，在土壤深度为10-20 cm和20-30 cm时，NT的累积CO ₂ -C排放量比RT高13％，但是RT的累积CO ₂ -C排放量比NT高10％和38％。分别。在土壤深度为0-10、10-20、20-30和30-50 cm时，BE的微生物商（M q）分别增加了近47％，70％，24％和167％。M q增加显着降低了SOC和某些碳分数。值得注意的是，在两种耕作制度下，BE均未改变整个土壤剖面中的DOC。在两个耕作系统中，BE在30-50 cm的土壤深度处的MBC均显着高于WA。在土壤深度为20-30 cm时，RT的MBC显着高于NT（4％）。总体而言，从土壤中添加BE中的DOC可通过修饰Mq来增强C矿化作用。