Four new daphniphyllum alkaloids, namely daphnicalycines A–D (1–4), together with nineteen known related ones (5–7 and 11–26), were isolated from the stems and leaves of Daphniphyllum calycinum. Their structures including the absolute configurations were unambiguously elucidated by extensive spectroscopic analysis, X-ray diffraction analysis and comparison with the reported data. In particular, with the assistance of X-ray diffraction analysis, we were able not only, for the first time, to give full ¹H and ¹³C NMR assignment of daphniteijsmanine (7) and to elucidate its absolute stereochemistry, but also lead to correct the structure of caldaphnidine E (9). The confusions reported for 1 and daphnimacropine (8) in the literature were also clarified.

四个新虎皮生物碱，即daphnicalycines A-d（1 - 4），加上19名已知的相关者（5 - 7和11 - 26），分离从茎叶虎皮瓣花。通过广泛的光谱分析，X射线衍射分析以及与报道数据的比较，明确阐明了它们的结构，包括绝对构型。特别是，借助X射线衍射分析，我们不仅能够首次给出daphniteijsmanine的全部¹ H和¹³ C NMR赋值（7）并阐明其绝对立体化学，也可导致校正卡尔达啡定E（9）的结构。还澄清了文献中报道的1和daphnimacropine（8）的混淆。