A red-emitting cyclometalated Ir(III) complex-based phosphorescent probe 1 was designed and synthesized to selectively and sensitively detect thiophenol in 90% DMSO/PBS (0.01 M, pH = 7.4) solution with a low detection limited (LOD = 8.68 × 10⁻⁷ mol L⁻¹). For probe 1, cyclometalated [Ir(ppy)₂Phen]⁺ moiety acted as phosphor, 2,4-dinitrobenzenesulfonyl (DNBS) group was regarded as recognition site and phosphorescent quencher, and polyether (1-ethoxy-2-(2-methoxyethoxy)ethane) units in the C^N ligands was introduced to improve the water solubility of complex. Due to strong electro-withdrawing DNBS moiety, probe 1 exhibited very week phosphorescence at 600 nm. Upon addition with thiophenol to probe 1 and DNBS moiety left due to the cleavage of sulfonamide bond though nucleophilic elimination reaction, an obvious phosphorescence at 600 nm belong to complex 2 could be observed and the solution displayed red phosphorescent color. Meanwhile, the probe possessed a large Stokes shift (180 nm) and a relatively larger phosphorescence lifetime (τ = 5.265 μs). At the same time, the probe could be used for quantitative detection of thiophenol in actual water samples with high recovery, and detect thiophenol in human serum (100-fold dilution by PBS).

设计并合成了一种发射红色的基于环金属化的Ir（III）配合物的磷光探针1，以选择性和灵敏的方式检测90％DMSO / PBS（0.01 M，pH = 7.4）溶液中的硫酚，且检测限低（LOD = 8.68× 10 ^-7  mol L ^-1）。对于探针1，环金属化的[Ir（ppy）₂ Phen] ⁺部分充当磷光体，2,4-二硝基苯磺酰基（DNBS）基团被视为识别位点和磷光猝灭剂，以及聚醚（1-乙氧基-2-（2-甲氧基乙氧基）引入C ^ N配体中的）乙烷单元以改善配合物的水溶性。由于具有强大的吸电DNBS部分，探针1在600 nm处表现出非常一周的磷光。在通过亲核消除反应将磺酰胺键裂解后，向探针1和残留的DNBS部分添加硫酚后，在600 nm处可见明显的磷光，属于配合物2，溶液呈红色磷光。同时，该探针具有较大的斯托克斯位移（180 nm）和相对较大的磷光寿命（τ= 5.265μs）。同时，该探针可用于定量回收高回收率的实际水样中的苯酚，并检测人血清中的苯酚（用PBS稀释100倍）。