Journal of Neuroscience Methods ( IF 2.7 ) Pub Date : 2020-10-03 , DOI: 10.1016/j.jneumeth.2020.108936 Ana Paula de Castro Araujo 1 , Jalles Dantas de Lucena 2 , Davi Carvalho Drieskens 3 , Lívia Rodrigues Neves 3 , Karen Cristina Pugliane 1 , Hindiael Aeraf Belchior 4 , Rochele Castelo-Branco 1 , Flávio Freitas Barbosa 1
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Background
Episodic-like memory tasks based on the spontaneous exploration of objects are commonly applied in one-trial protocols. However, multiple-trial designs are known to reduce animal numbers and data variance, providing faster accumulation of data.
New method
In this study, we devised a new object recognition memory task for rats that carry out multiple trials per session. We developed three types of continual trial tasks: a longer protocol, a shorter protocol, and a protocol in which the experimental session was divided into two days.
Results
In our design, rats expressed temporal and spatial memory, but not what-where-when content integration. We found that shorter protocols were more efficient to evaluate memory capabilities.
Comparison with existing methods
To the best of our knowledge, it is the first object recognition task with multiple trials that simultaneously assess the temporal and spatial aspects of episodic-like memory.
Conclusions
We suggest that our task is suitable for the simultaneous measurements of brain functions related to spatial and temporal attributes in rats.
中文翻译：
大鼠通过多次试验识别新对象识别记忆任务中的时空属性
背景
基于对象自发探索的类似情景的记忆任务通常用于一审协议。但是，已知多种试验设计可以减少动物数量和数据差异，从而提供更快的数据积累。
新方法
在这项研究中，我们为大鼠设计了一种新的对象识别记忆任务，该任务每节进行多次试验。我们开发了三种类型的连续试用任务：较长的协议，较短的协议以及将实验时间分为两天的协议。
结果
在我们的设计中，大鼠表达了时空记忆，但没有表达内容整合的位置。我们发现较短的协议可以更有效地评估内存功能。
与现有方法的比较
据我们所知，这是第一个具有多个试验的对象识别任务，该试验同时评估情节式记忆的时间和空间方面。
结论
我们建议我们的任务适合同时测量与大鼠的时空属性有关的脑功能。
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