Perkinsus spp. parasites have significant impact on aquaculture and wild mollusc populations. We sequenced the genomes of five monoclonal isolates of Perkinsus olseni and one Perkinsus chesapeaki from international sources. Sequence analysis revealed similar levels of repetitive sequence within species, a polyploid genome structure, and substantially higher heterozygosity in Oceanian-sourced isolates. We also identified tandem replication of the rRNA transcriptional unit, with high strain variation. Characterized gene content was broadly similar amongst all Perkinsus spp. but P. olseni Oceanian isolates contained an elevated number of genes compared to other P. olseni isolates and cox3 could not be identified in any Perkinsus spp. sequence. Phylogenetics and average nucleotide identity scans were consistent with all P. olseni isolates being within one species. These are the first genome sequences generated for both P. olseni and P. chesapeaki and will allow future advances in diagnostic design and population genomics of these important aquatic parasites.

Perkinsus spp。寄生虫对水产养殖和野生软体动物种群有重大影响。我们对来自国际来源的五种Perkinsus olseni单克隆分离株和一种Perkinsus chesapeaki的基因组进行了测序。序列分析揭示了物种内相似水平的重复序列、多倍体基因组结构以及大洋洲来源的分离株中显着更高的杂合性。我们还鉴定了 rRNA 转录单位的串联复制，具有高应变变异。所有Perkinsus spp的特征基因含量大致相似。但与其他P. olseni分离株相比，P. olseni大洋洲分离株含有更多的基因，并且cox3在任何Perkinsus spp中都无法识别。顺序。系统发育和平均核苷酸同一性扫描与所有P. olseni分离株在一个物种内一致。这些是为P. olseni和P. chesapeaki生成的第一个基因组序列，并将允许这些重要水生寄生虫的诊断设计和种群基因组学的未来进展。