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Genotypic and phenotypic diversity among Lactobacillus plantarum and Lactobacillus pentosus isolated from industrial scale cucumber fermentations
Food Microbiology ( IF 5.3 ) Pub Date : 2020-10-04 , DOI: 10.1016/j.fm.2020.103652
Ilenys M. Pérez-Díaz , Suzanne D. Johanningsmeier , Kartheek Anekella , Christian G. Pagán-Medina , Lesley Méndez-Sandoval , Consuelo Arellano , Robert Price , Katheryne V. Daughtry , Michelle Borges , Chloe Bream , Lauren Connelly , Susan E. Dieck , Meredith T. Levi , Erin K. McMurtrie , Rickey E. Smith , Jeannette C. Theora , Paige Wendland , Francisco Gómez-Rodríguez , Francisco Noé Arroyo-López

The Lactobacillus plantarum and Lactobacillus pentosus genotypes existing in industrial-scale cucumber fermentations were defined using rep-PCR-(GTG)5. The ability of each genotype to ferment cucumbers under various conditions was evaluated. Rep-PCR-(GTG)5 was the technique capable of illustrating the most intraspecies discrimination compared to the sequencing of housekeeping genes (recA, dnaK, pheS and rpoA), MLST and RAPD with primers LP1, OPL5, M14 and COC. Ten genotypic clusters were defined for the 199 L. pentosus tested and three for the 17 L. plantarum clones. The ability of the 216 clones genotyped and 37 additional cucumber fermentation isolates, of the same species, to rapidly decrease the pH of cucumber juice medium under various combinations of sodium chloride (0 or 6%), initial pH (4.0 or 5.2) and temperatures (15 or 30 °C) was determined using a fractional factorial screening design. A reduced fermentation ability was observed for the L. plantarum strains as compared to L. pentosus, except for clone 3.2.8, which had a ropy phenotype and aligned to genotypic cluster A. L. pentosus strains belonging to three genotypic clusters (B, D and J) were more efficient in cucumber juice fermentation as compared to most L. plantarum strains. This research identified three genetically diverse L. pentosus strains and one L. plantarum as candidates for starter cultures for commercial cucumber fermentations.



中文翻译:

从工业规模黄瓜发酵中分离的植物乳杆菌戊糖乳杆菌的基因型和表型多样性

植物乳杆菌戊糖乳杆菌存在于工业规模的发酵黄瓜基因型使用Rep-PCR-(GTG)5限定。评估了每种基因型在各种条件下发酵黄瓜的能力。与管家基因(recA,dnaK,pheSrpoA),MLST和RAPD的引物LP1,OPL5,M14和COC的测序相比,Rep-PCR-(GTG)5是一种能够说明大多数种内歧视的技术。对199个戊氏乳杆菌定义了10个基因型簇,对17个植物乳杆菌定义了3个基因型簇克隆。基因型的216个克隆和37个其他黄瓜发酵菌株的基因分型在氯化钠(0或6%),初始pH(4.0或5.2)和温度的各种组合下迅速降低黄瓜汁培养基的pH值的能力(15或30°C)使用分数阶数筛选设计确定。与戊糖乳杆菌相比,植物乳杆菌的发酵能力有所降低,但克隆3.2.8除外,该克隆具有显性表型并与基因型簇A对齐。戊糖乳杆菌菌株属于三个基因型簇(B,与大多数植物乳杆菌相比,D和J)在黄瓜汁发酵中更有效株。这项研究确定了三种遗传上不同的戊糖乳杆菌菌株和一种植物乳杆菌作为商业黄瓜发酵的起始培养的候选对象。

更新日期:2020-10-15
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