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Point-of-care multiplex-PCR enables germ identification in haemolytic uremic syndrome 94 h earlier than stool culture
European Journal of Clinical Microbiology & Infectious Diseases ( IF 4.5 ) Pub Date : 2020-10-04 , DOI: 10.1007/s10096-020-04060-x
Luis Hernán Llano López 1 , Pablo Melonari 2 , Stephan Gehring 3 , Daniel Schreiner 3 , Sandra Grucci 4 , Sofía Pérez Araujo 5 , Lorena Di Pauli 2 , Christina Oetzmann von Sochaczewski 6 , Arne Schröder 7 , Laura Piovano 1
Affiliation  

Haemolytic uremic syndrome often affects children causing a relevant morbidity and mortality. We compared the time to diagnosis by multiplex-PCR and stool culture in 15 children from two centres. Multiplex-PCR accelerated the time to diagnosis by 94 (95% confidence interval, 80–119; P = 0.0007) hours. Multiplex-PCR offers a time advantage of stool culture that may aid in earlier identification of outbreak clusters.



中文翻译:

护理点多重 PCR 能够比粪便培养早 94 小时识别溶血性尿毒症综合征的细菌

溶血性尿毒症综合征经常影响儿童,导致相关的发病率和死亡率。我们比较了来自两个中心的 15 名儿童通过多重 PCR 和粪便培养的诊断时间。多重 PCR 将诊断时间缩短了 94(95% 置信区间,80-119;P  = 0.0007)小时。多重 PCR 提供了粪便培养的时间优势,可能有助于早期识别爆发群。

更新日期:2020-10-04
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