This study aims to explore the role of Gastrokin-2 (GKN2) in gastric cancer and its function in the progression and metastasis of gastric cancer. The expression of GKN2 in the patient samples was examined by qRT-PCR and western blot. The transcription factor NK6 Homeobox 2 (NKX6-2), which binds to the GKN2 promoter, was predicted by cBioportal and JSPAR. Binding between NKX6-2 and the GKN2 promoter was analyzed by dual-luciferase assay. MTT assay and transwell assay were used to detect changes in gastric cancer cell viability and migration after GKN2 overexpression, which was achieved by transfection of GKN2 overexpression vector. Akt signaling pathway markers were assessed by western blot. GKN2 is downregulated in gastric cancer and low GKN2 expression is correlated to poor survival, metastasis, and higher clinical stages. NKX6-2 binds the promoter region of GKN2 and regulate its expression. GKN2 overexpression inhibits the proliferation, migration, and invasion of gastric cancer cells, which was mediated by Akt signaling pathway. NKX6-2 regulated GKN2 inhibits the proliferation and invasion of gastric cancer cells by inhibiting Akt signaling pathway. GKN2 can be used as a potential diagnostic and therapeutic target for patients with clinical gastric cancer.

本研究旨在探讨Gastrokin-2（GKN2）在胃癌中的作用及其在胃癌进展和转移中的作用。通过qRT-PCR和western blot检查患者样品中GKN2的表达。cBioportal和JSPAR预测了与GKN2启动子结合的转录因子NK6 Homeobox 2（NKX6-2）。通过双荧光素酶测定法分析了NKX6-2和GKN2启动子之间的结合。MTT法和transwell法用于检测GKN2过表达后胃癌细胞活力和迁移的变化，这是通过转染GKN2过表达载体实现的。通过蛋白质印迹评估Akt信号通路标记。GKN2在胃癌中被下调，并且低GKN2表达与不良的生存，转移和更高的临床阶段相关。NKX6-2结合GKN2的启动子区域并调节其表达。GKN2过表达抑制胃癌细胞的增殖，迁移和侵袭，这是由Akt信号通路介导的。NKX6-2调节的GKN2通过抑制Akt信号通路来抑制胃癌细胞的增殖和侵袭。GKN2可用作临床胃癌患者的潜在诊断和治疗靶标。