The incidence of cancer is increasing worldwide; likewise, the emergence of antibiotic-resistant biofilm-forming pathogens has led to a tremendous increase in morbidity and mortality. This study aimed to evaluate the probiotic properties of bacteriocin-producing Enterococcus sp. with a focus on their anti-biofilm and anticancer activities. Three of 79 Enterococcus isolates (FM43, FM65, FM50) were identified as producers of broad-spectrum bioactive molecules and were molecularly characterized as Enterococcus faecium by 16S rRNA sequencing. Phenotypic and genotypic screening for potential virulence factors revealed no factors known to promote pathogenicity. Treatment with proteinase K resulted in diminished antimicrobial activity; PCR-based screening for bacteriocin genes suggested the presence of both entA and entB genes that encode enterocins A and B, respectively. Maximum antimicrobial activity was detected during the early stationary phase, while activity disappeared after 24 h in culture. Bacteriocins from these isolates were stable at high temperatures and over a wide range of pH. Interestingly, crude supernatants of Ent. faecium FM43 and Ent. faecium FM50 resulted in significant destruction (80% and 48%, respectively; P < 0.05) of Streptococcus mutans ATCC 25175–associated preformed biofilms. Moreover, in vitro cytotoxicity assays revealed that extracts from Ent. faecium isolates FM43, FM65, and FM50 inhibited Caco-2 cell proliferation by 76.9%, 70%, and 85.3%, respectively. Taken together, the multifunctional capabilities of the microbial-derived proteins identified in our study suggest potentially important roles as alternative treatments for biofilm-associated infections and cancer.

世界范围内癌症的发病率正在上升；同样，耐药性生物膜形成病原体的出现导致发病率和死亡率大幅增加。本研究旨在评估产细菌素肠球菌的益生菌特性。重点是它们的抗生物膜和抗癌活性。79株肠球菌中的 3株（FM43、FM65、FM50）被鉴定为广谱生物活性分子的生产者，并被鉴定为屎肠球菌通过 16S rRNA 测序。潜在毒力因子的表型和基因型筛选显示没有已知的促进致病性的因素。用蛋白酶 K 处理导致抗菌活性降低；基于 PCR 的细菌素基因筛选表明存在entA和entB基因，分别编码肠素 A 和 B。在早期稳定期检测到最大的抗菌活性，而在培养 24 小时后活性消失。来自这些分离株的细菌素在高温和很宽的 pH 范围内是稳定的。有趣的是，Ent 的粗上清液。屎肠球菌FM43和耳鼻喉科。粪便FM50 导致显着破坏（分别为 80% 和 48%；P < 0.05)变形链球菌ATCC 25175 相关的预制生物膜。此外，体外细胞毒性试验表明，Ent. 的提取物。肠球菌分离物FM43，FM65，FM50和分别为76.9％，70％，和85.3％，抑制了Caco-2细胞的增殖。综上所述，我们的研究中鉴定的微生物衍生蛋白质的多功能能力表明作为生物膜相关感染和癌症的替代疗法具有潜在的重要作用。