Japanese encephalitis is a vector borne disease and the best procedure for control of this disease is control of vector population. Now a day use of phyto-chemicals more preferred over use of chemical insecticides. Isolation, purification and determination of chemical structures of bioactive compounds were done successfully from n-hexane extract of leaf of M. ferrea. The compounds shows better potentiality to control Cx. vishnui 3rd instars larvae. n-hexane extract of leaf of M. ferrea was prepared by Sohxlet apparatus and further used for bioassay against 3rd instars larvae of Cx. vishnui. n-hexane extract was further fractioned by Thin layer chromatography to isolate bioactive compounds. From different band of TLC, bioactive compounds bearing band was isolated by bioassay test. Structures of active principles were determined by FTIR, gas chromatography, mass spectrometry and with the help of library data list. Cent percent mortality of Cx. vishnui larvae was recorded at 45 ppm concentration of n-hexane extract of M. ferrea after 72 h of exposure. In isolated bioactive compounds 3-(2-Hydroxyphenoxy)-1, 2-propanediol is one of predominant compound, may be responsible for mortality of Cx. vishnui larvae. LC50 value for Cx. vishnui 3rd instars larvae of active compound are 26.76, 25.64 and 20.32 ppm for 24 h, 48 h and 72 h respectively. No mortality were recorded when sub lethal concentration (LC50) of 24 h of bioactive compound applied on non-target organisms i.e. Chironomus circumdatus, Daphnia, Diplonychus etc. The compound i.e. 3-(2-Hydroxyphenoxy)-1, 2-propanediol is predominant compound of active principles and its chemical structure is determine which is major component of n-hexane extract of leaf of M. ferrea. These bioactive principles are safe for other organisms (non-target) those share the same natural habitat.

中文翻译：

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通过从 Mesua ferrea Linn 叶的正己烷提取物中分离的活性成分 3-(2-Hydroxyphenoxy)-1, 2-丙二醇控制日本脑炎。

日本脑炎是一种媒介传播疾病，控制这种疾病的最佳程序是控制媒介种群。现在每天使用植物化学物质比使用化学杀虫剂更受欢迎。从M. ferrea叶的正己烷提取物中成功地分离、纯化和测定了生物活性化合物的化学结构。这些化合物显示出更好的控制 Cx 的潜力。vishnui 3 龄幼虫。用Sohxlet 装置制备了M. ferrea 叶的正己烷提取物，并进一步用于抗Cx 3 龄幼虫的生物测定。毗湿奴。通过薄层色谱法进一步分离正己烷提取物以分离生物活性化合物。从薄层色谱的不同条带中，通过生物测定试验分离出带有生物活性化合物的条带。有效成分的结构由 FTIR 确定，气相色谱、质谱和库数据列表的帮助。Cx 的百分比死亡率。暴露 72 小时后，在 45 ppm 浓度的 M. ferrea 正己烷提取物中记录了 vishnui 幼虫。在分离的生物活性化合物 3-(2-Hydroxyphenoxy)-1 中，2-丙二醇是主要化合物之一，可能是导致 Cx 死亡的原因。毗湿奴幼虫。Cx 的 LC50 值。vishnui 3 龄幼虫活性化合物在24 h、48 h 和72 h 分别为26.76、25.64 和20.32 ppm。当生物活性化合物以 24 小时的亚致死浓度 (LC50) 应用于非靶标生物，即圆纹摇蚊、水蚤、双爪属等时，未记录到死亡。该化合物即 3-(2-羟基苯氧基)-1，2-丙二醇是活性成分的主要化合物，其化学结构决定了马齿苋叶正己烷提取物的主要成分。这些生物活性成分对于共享相同自然栖息地的其他生物（非目标）是安全的。