In this study, the Bacillus subtilis T4b xylanase was characterized in terms of optimal catalytic activity and production culture medium based on agro-industrial by-products using response surface methodology (RSM). The xylan concentration, pH, and temperature were statistically optimized to achieve the highest xylanase activity. The most suitable combination of variables for higher xylanase activity (412 U/mL) was 7.07%, 6.26, and 48.96 °C for substrate concentration, pH, and temperature, respectively. The stability of partially purified xylanase was highlighted at a pH range of 5.0–10.0 and a temperature of 40 °C for 48 h. A significant improvement in xylanase activity was found by adding NaCl (up to 50 mM) and 5% ethanol to the substrate. A molecular mass of 35.0 kDa was demonstrated for partially purified xylanase on SDS-polyacrylamide gel. An economical culture medium based on novel substrates including canola meal and tomato pomace screened among different agro-industrial by-products to replace the high-cost ingredients in the defined enzyme production medium. To maximize the enzyme’s yield in the newly developed culture medium, statistical optimization of final selected ingredient concentrations was done using RSM. An optimized medium resulted in more than threefold higher xylanase production. It could be concluded that the B. subtilis T4b xylanase is an interesting option to develop the bioconversion of valuable by-products such as tomato pomace to high value-added products via suitable enzymatic hydrolysis.

在这项研究中，枯草芽孢杆菌T4b木聚糖酶的特征在于使用响应表面方法（RSM）基于农业工业副产物的最佳催化活性和生产培养基。对木聚糖浓度，pH和温度进行统计优化，以实现最高的木聚糖酶活性。对于较高的木聚糖酶活性（412 U / mL），最合适的变量组合分别是底物浓度，pH和温度分别为7.07％，6.26和48.96°C。在5.0–10.0的pH范围和40°C的温度下48小时，突出显示了部分纯化的木聚糖酶的稳定性。通过向底物中添加NaCl（最高50 mM）和5％的乙醇，发现木聚糖酶活性有了显着改善。在SDS-聚丙烯酰胺凝胶上，部分纯化的木聚糖酶的分子量为35.0 kDa。一种经济的培养基，基于油菜籽粉和番茄渣等新型底物，在不同的农业工业副产品中进行了筛选，以替代确定的酶生产培养基中的高价成分。为了在新开发的培养基中最大化酶的产量，使用RSM对最终选定成分的浓度进行了统计优化。优化的培养基可使木聚糖酶产量提高三倍以上。可以得出结论，优化的培养基可使木聚糖酶产量提高三倍以上。可以得出结论，优化的培养基可使木聚糖酶产量提高三倍以上。可以得出结论，枯草芽孢杆菌T4b木聚糖酶是通过有价值的酶水解将有价值的副产物（例如番茄渣）生物转化为高附加值产品的有趣选择。