Pentachlorophenol (PCP) is a recalcitrant biocide that bioaccumulates in the environment due to its persistent nature and has been listed as a priority pollutant due to its toxicological and health effects. In this study, a novel PCP-degrading Bacillus cereus strain AOA-CPS1 (BcAOA) was isolated from wastewater and characterized for PCP biotransformation in a batch reactor. The degradation kinetics were elucidated via substrate inhibition models, while PCP biotransformation was established by spectrophotometric and GC–MS analysis. BcAOA shared 95% sequence homology with Bacillus cereus strain XS2 and is closely related to some B. cereus strains which are previously reported to degrade PCP and other related pollutants. BcAOA degraded 74% of 350 mg l⁻¹ of PCP within 9 days in a batch culture. The biotransformation of PCP by BcAOA followed the first and zero-order kinetics at low and high PCP concentration, respectively, with biokinetic constants: maximum biotransformation rate (0.0996 mg l⁻¹ h⁻¹); substrate inhibition constant (723.75 mg l⁻¹); half-saturation constant (171.198 mg l⁻¹) and R² (0.98). The genes (pcpABCDE, cytochrome P450) encoding the enzymes involved in the biodegradation of PCP were amplified from the genomic DNA of BcAOA. Further, depending upon the genes amplified and identified metabolites using GC–MS, two different PCP biotransformation pathways were proposed in this study. Cloning and expression of the catabolic genes are underway to map out the concise pathway for PCP biotransformation by BcAOA.

五氯苯酚（PCP）是顽固的杀生物剂，由于其持久的性质而在环境中生物累积，由于其毒理学和健康影响，已被列为优先污染物。在这项研究中，从废水中分离出了一种新型的降解PCP的蜡样芽孢杆菌AOA-CPS1菌株（Bc AOA），并对其进行了批处理反应器中PCP生物转化的表征。通过底物抑制模型阐明了降解动力学，同时通过分光光度法和GC-MS分析确定了PCP的生物转化。Bc AOA与蜡状芽孢杆菌XS2菌株具有95％的序列同源性，并且与某些蜡状芽孢杆菌密切相关以前据报道会降解PCP和其他相关污染物的菌株。 在分批培养中，BcAOA在9天内降解了350 mg ^-1的PCP中的74％。在低和高PCP浓度下，Bc AOA对PCP的生物转化分别遵循一阶和零阶动力学，且具有生物动力学常数：最大生物转化速率（0.0996 mg l ^-1  h ^-1）；底物抑制常数（723.75 mg l ^-1）；半饱和常数（171.198 mg l ^-1）和R ²（0.98）。从Bc的基因组DNA中扩增出编码与PCP生物降解有关的酶的基因（pcp ABCDE，细胞色素P450）。AOA。此外，根据使用GC-MS扩增和鉴定的代谢物的基因，本研究提出了两种不同的PCP生物转化途径。分解代谢基因的克隆和表达正在进行中，以描绘出由Bc AOA进行PCP生物转化的简洁途径。