Probing cell membrane damage using a molecular rotor probe with membrane-to-nucleus translocation,Materials Horizons

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Probing cell membrane damage using a molecular rotor probe with membrane-to-nucleus translocation
Materials Horizons ( IF 12.2 ) Pub Date : 2020-10-02 , DOI: 10.1039/d0mh01141j
Kang-Nan Wang _{1,

2,

3,

4,

5} , Guobin Qi _{4,

5,

6,

6} , Huiying Chu _{7,

8,

9,

10,

11} , Xi-Juan Chao _{1,

2,

3} , Liu-Yi Liu _{1,

2,

3} , Guohui Li _{7,

8,

9,

10,

11} , Qian Cao _{1,

2,

3} , Zong-Wan Mao _{1,

2,

3} , Bin Liu _{4,

5,

6,

6,

12}

Affiliation

Damage to cell membranes, the outermost protection layer, is fatal to cells. However, precisely monitoring and in situ reporting cell membrane damage is not trivial. Herein, we present a molecular rotor probe, TPAE2, which can effectively bind to DNA and 1,2-dioleoyl-sn-glycero-3-phosphocholine in solution. Due to the light-up imaging characteristics of the molecular rotor, TPAE2 offers ultrafast and wash-free staining of plasma membrane with 160-fold fluorescence “turn-on” and excellent photostability. Once the membrane is damaged, TPAE2 can light-up the nucleus as a signal reporter. The cascade imaging of the cell membrane and nucleus using TPAE2 enabled real-time tracking of the whole process of cell apoptosis. What's more, under irradiation, TPAE2 stained on the cell membrane could penetrate cells rapidly and selectively stain the nucleus, self-reporting the cancer cell ablation process. This is the first example that a single molecule with multiple functions can light up the nucleus as an indication of cell membrane damage. The membrane-to-nucleus translocation strategy opens up a new avenue for the design of membrane damage diagnosis probes for biomedical applications.

中文翻译：

使用具有膜到核易位的分子转子探针探测细胞膜损伤

细胞膜的最外层保护层受损，对细胞是致命的。但是，精确监测和原位报告细胞膜损伤并非易事。在本文中，我们提出了一种分子转子探针TPAE2，它可以有效地结合DNA和溶液中的1,2-二油酰基-sn-甘油-3-磷酸胆碱。由于分子转子具有发光成像特性，TPAE2可对质膜进行超快速免洗染色，并具有160倍的荧光“开启”和出色的光稳定性。膜受损后，TPAE2可以照亮细胞核，作为信号报告基因。使用TPAE2对细胞膜和细胞核进行级联成像实时跟踪细胞凋亡的全过程。更重要的是，在辐射下，染色在细胞膜上的TPAE2可以迅速穿透细胞并选择性地染色细胞核，从而自我报告癌细胞的消融过程。这是第一个具有多个功能的分子可以点亮细胞核以指示细胞膜损伤的例子。膜到核的移位策略为生物医学应用的膜损伤诊断探针的设计开辟了一条新途径。

更新日期：2020-11-03

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