The Colorado potato beetle (CPB) is a prominent insect pest of potatoes, tomatoes and eggplants all over the world, however, the management of CPB remains a challenging task for more than one hundred years. We have successfully developed bacteria-expressed dsRNA-mediated feeding RNA interference (RNAi) approach in our previous study. A critical step towards field management of CPB via feeding RNAi is to identify effective and environmentally safe target genes. NADPH-Cytochrome P450 reductase (CPR) plays a central role in cytochrome P450 action. The full length Leptinotarsa decemlineata CPR (LdCPR) cDNA was isolated from an imidacloprid resistant population. The LdCPR gene was ubiquitously expressed in all stages tested but showed an increase in expression during the early stage of embryonic development. The bacteria-expressed dsRNA-mediated feeding RNAi of LdCPR in adults caused systemic knock down expression of the gene coding for LdCPR in both adults and their eggs. Suppression of LdCPR expression increased susceptibility of imidacloprid in resistant beetles, as well as a significant decrease of fecundity in female beetles (29% less eggs/day) and the hatching rate (47%) of their eggs. These data suggest that LdCPR plays important roles in insecticide detoxification and biosynthetic pathways of endogenous compounds and may serve as an essential target to control CPB.

中文翻译：

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沉默NADPH-细胞色素P450还原酶影响吡虫啉的吡虫啉敏感性，繁殖力和胚胎发育

科罗拉多马铃薯甲虫（CPB）是全世界马铃薯，西红柿和茄子的主要害虫，但是，一百多年来，CPB的管理仍然是一项艰巨的任务。在先前的研究中，我们已经成功开发了细菌表达的dsRNA介导的喂养RNA干扰（RNAi）方法。通过饲喂RNAi进行CPB现场管理的关键步骤是鉴定有效且对环境安全的靶基因。NADPH细胞色素P450还原酶（CPR）在细胞色素P450的作用中起着核心作用。从吡虫啉抗性种群中分离了全长小球藻（Leptinotarsa decemlineata）CPR（LdCPR）cDNA。LdCPR基因在所有测试阶段均普遍表达，但在胚胎发育的早期阶段表达增加。成年人中由细菌表达的dsRNA介导的LdCPR喂养RNAi导致成年人及其卵中编码LdCPR的基因的系统敲低表达。LdCPR表达的抑制增加了吡虫啉在抗性甲虫中的易感性，以及雌性甲虫的繁殖力显着降低（每天减少29％的卵）和卵的孵化率（47％）。这些数据表明，LdCPR在杀虫剂解毒和内源性化合物的生物合成途径中起着重要作用，并可能成为控制CPB的重要靶标。以及雌性甲虫的繁殖力显着降低（每天减少29％的卵）和卵的孵化率（47％）。这些数据表明，LdCPR在杀虫剂解毒和内源性化合物的生物合成途径中起着重要作用，并可能成为控制CPB的重要靶标。以及雌性甲虫的繁殖力显着降低（每天减少29％的卵）和卵的孵化率（47％）。这些数据表明，LdCPR在杀虫剂解毒和内源性化合物的生物合成途径中起着重要作用，并可能成为控制CPB的重要靶标。