Abstract This study aimed to compare the formation of polymicrobial biofilms using carious dentin or saliva as inoculum for application in in vitro microbiological studies on caries research. For biofilm growth, combined samples of infected dentin or saliva from three donors were used. The biofilms were grown on glass coverslips, under a regimen of intermittent exposure (6 h day−1) to 1% sucrose for 4 days. Total bacterial loads, as well as specific aciduric bacteria and mutans streptococci loads were quantified and correlated with biofilm acidogenicity and susceptibility to chlorhexidine. The data were evaluated using the Student’s-t, Mann Whitney and Kruskal-Wallis tests. The two biofilms showed similar microbial loads (total bacteria, aciduric bacteria and mutans streptococci) on day 4, and high acidogenicity after 48 h and were susceptible to chlorhexidine at different time intervals. In conclusion, both dentin and saliva can be used as an inoculum in in vitro studies of processes related to biofilm formation.

中文翻译：

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从唾液或牙本质龋损中获得的多微生物生物膜的表征

摘要 本研究旨在比较使用龋牙本质或唾液作为接种物的多微生物生物膜的形成，以用于龋病研究的体外微生物研究。对于生物膜生长，使用了来自三个供体的受感染牙本质或唾液的组合样本。生物膜在玻璃盖玻片上生长，采用间歇性暴露（6 h day-1）1% 蔗糖 4 天的方案。总细菌负荷，以及特定的酸尿菌和变形链球菌负荷被量化，并与生物膜产酸性和对氯己定的敏感性相关联。使用Student's-t、Mann Whitney 和Kruskal-Wallis 检验评估数据。两种生物膜在第 4 天显示相似的微生物负荷（总细菌、酸尿菌和变形链球菌），48 小时后具有高产酸性，并且在不同的时间间隔对氯己定敏感。总之，在与生物膜形成相关的过程的体外研究中，牙本质和唾液都可以用作接种物。