Disease resistance (R) gene, RPP13, plays an important role in the resistance of plants to pathogen infections; its function in resistance of wheat to powdery mildew remains unknown. In this study, a RNA‐Seq technique was used to monitor expression of genes in susceptible wheat ‘Jing411’ and resistant near‐isogenic line ‘BJ‐1’ in response to powdery mildew infection. Overall, 413 differential expression genes were observed and identified as involved in disease resistance. RPP13 homologous gene on wheat chromosome 7D was preliminarily identified using the wheat 660K SNP chip. RPP13 was highly expressed in ‘BJ‐1’ and encodes 1,027 amino acids, including CC, NB and LRR domain, termed TaRPP13‐3. After inoculation with powdery mildew, expression of TaRPP13‐3 in resistant wheat changed with time, but average expression was higher when compared to susceptible variety, thus indicating that TaRPP13‐3 is involved in resistance to powdery mildew. Virus‐induced gene silencing (VIGS) was used to inhibit expression of TaRPP13‐3 in resistant parent ‘Brock’. Results indicated that silencing of TaRPP13‐3 led to decreased disease resistance in ‘Brock’. Overall results of this study indicate that TaRPP13‐3 gene is involved in the defence response of wheat to powdery mildew and plays a positive role in wheat powdery mildew interactions.

中文翻译：

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TaRPP13-3是位于chr 7D的类似CC-NBS-LRR的基因，可增强Brock对小麦白粉病的抗病性

疾病抗性（R）基因RPP13在植物对病原体感染的抗性中起重要作用。其在小麦抗白粉病中的作用尚不清楚。在这项研究中，RNA-Seq技术用于监测易感小麦'Jing411'和抗近等基因系'BJ-1'中白粉病感染的基因表达。总体上，观察到并鉴定出413个差异表达基因与疾病抗性有关。使用小麦660K SNP芯片初步鉴定了小麦7D染色体上的RPP13同源基因。RPP13在'BJ-1'中高表达，编码1,027个氨基酸，包括CC，NB和LRR结构域，称为TaRPP13-3。接种白粉病后，抗性小麦中TaRPP13-3的表达随时间变化，但与易感品种相比，平均表达较高，这表明TaRPP13-3参与了白粉病的抗性。病毒诱导的基因沉默（VIGS）被用来抑制TaRPP13-3在抗性亲本'Brock'中的表达。结果表明，沉默TaRPP13-3会导致'Brock'的抗病性下降。这项研究的总体结果表明，TaRPP13-3基因参与了小麦对白粉病的防御反应，并在小麦白粉病相互作用中发挥了积极作用。