Optical clearing skull window is a novel technique compared to the traditional surgery based cranial window, which could reduce the scattering of skull and make it transparent to visible light. Here, the NIR‐II optimized skull clearing window was established. In the application of NIR‐II excited THG microscopy, the imaging depth was more than 3 times as it without optical clearing, and close to it with the open‐skull window. In addition, the established skull optical clearing window promises to realize NIR‐II laser induced targeted injury of cortical singlevessel.

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与传统的基于颅骨手术的颅窗相比，光学清除颅骨窗是一种新颖的技术，可以减少颅骨的散射并使它对可见光透明。在这里，建立了NIR-II优化的颅骨清除窗口。在NIR-II激发THG显微镜的应用中，成像深度是未光学清除时的3倍以上，并通过开颅窗接近。此外，已建立的颅骨光学清除窗口有望实现NIR-II激光诱导的皮质单血管的靶向损伤。