The interface between pioneer sessile bacteria and a supporting substrate can be probed in situ and at the molecular scale by infrared spectroscopy in the attenuated total reflection mode (ATR‐FTIR). Here, a self‐assembled monolayer (SAM) of amino‐terminated alkanethiol is formed on the internal reflection element (IRE) composed of zinc selenide, and the attachment of model bacterium Lactobacillus rhamnosus GG (LGG) is subsequently studied. The impact of the beforehand surface preparation of the IRE on the SAM is studied on ZnSe substrates (i) cleaned by exposure to ozone/UV, (ii) acid cleaned, or (iii) coated with a thin gold film. The surface properties of the obtained substrates are analyzed by atomic force and electron microscopies, and elastic ion backscattering spectrometry. The kinetics of the formation and the organization of the formed SAMs are strongly surface dependent, as evidenced with ATR‐FTIR. Acid‐cleaned and gold‐coated IREs are the least and most favorable substrates for alkanethiol SAM formation, respectively. Regardless of differences in SAM properties, the average degree of LGG attachment is similar on all functionalized substrates. The molecular organization of LGG cells, however, is substrate‐dependent suggesting a possible effect of SAM organization on the bacteria–substrate interface.

中文翻译：

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硒化锌上烷硫醇自组装的原位监测：底物预处理的作用及其在细菌附着中的意义

可通过衰减全反射模式（ATR-FTIR）的红外光谱原位探测无柄细菌与支持底物之间的界面，并在分子尺度上进行探测。在此，在由硒化锌和模型细菌鼠李糖乳杆菌组成的内部反射元件（IRE）上形成了氨基末端链烷硫醇的自组装单分子层（SAM）。随后研究了GG（LGG）。在ZnSe衬底上研究了IRE的预先表面处理对SAM的影响（i）通过暴露在臭氧/紫外线下清洁，（ii）酸清洁，或（iii）涂有薄金膜。通过原子力和电子显微镜以及弹性离子背散射光谱法分析获得的基材的表面性质。正如ATR-FTIR所证明的那样，形成的SAM的形成和组织动力学在很大程度上取决于表面。酸洗和镀金的IREs分别是形成链烷硫醇SAM的最不理想的基材。无论SAM特性如何，LGG附着的平均程度在所有功能化基材上均相似。LGG细胞的分子组织，但是，