Abstract.

The conductive lipid pores occurring in planar bilayer membranes are known to manifest themselves experimentally as current fluctuations. Reliable recording of such fluctuations during phase transitions, as well as in membranes with various additives (for example, SDS), allows one to determine the characteristics of hypothetical hydrophilic pores, namely, their number, sizes, lifetimes, and duration of time intervals between pores. Because, in contrast with electroporation, the emergence of pores in a membrane does not require high voltages, this process is called soft poration. Studying the characteristics of pores under soft poration allows us to estimate the parameters of the Smoluchowski equation and compare them with the corresponding parameters used to describe electroporation. In this work, the experimental characteristics of current fluctuations in the membrane with the addition of SDS to the bulk solution were used to estimate the parameters of the Smoluchowski equation: the pore edge tension, the energy of the hydrophobic pore/hydrophilic pore barrier, the coefficient of pore diffusion in the radius space, the initial distribution density of the number of pores, and the attempt rate density of the lipids in a membrane. The obtained estimates are close to the parameter values used in studies of electroporation.

Graphical abstract

中文翻译：

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Smoluchowski方程参数的估计，该方程描述了软渗透条件下双层脂质膜中孔隙的出现

摘要。

已知存在于平面双层膜中的导电脂质孔在实验上表现为电流波动。可靠记录相变过程中以及含有各种添加剂（例如SDS）的膜中的此类波动，可以确定假设的亲水性孔的特征，即其数量，大小，寿命和之间的时间间隔持续时间毛孔。因为与电穿孔相反，膜中出现的孔不需要高压，所以此过程称为软渗透。研究软孔隙条件下的孔隙特征使我们能够估算Smoluchowski方程的参数，并将其与用于描述电穿孔的相应参数进行比较。在这项工作中 通过向本体溶液中添加SDS来研究膜中电流波动的实验特性，以估算Smoluchowski方程的参数：孔边缘张力，疏水性孔/亲水性孔屏障的能量，孔扩散系数在半径空间中，孔的数量的初始分布密度以及膜中脂质的尝试率密度。所得估计值接近电穿孔研究中使用的参数值。孔数的初始分布密度，以及膜中脂质的尝试率密度。所得估计值接近电穿孔研究中使用的参数值。孔数的初始分布密度，以及膜中脂质的尝试率密度。所得估计值接近电穿孔研究中使用的参数值。

图形概要