Abstract

We present simultaneous spatial and temporal polarization pulse shaping for two-photon excited fluorescence of dyes. A temporal pulse shaper and a subsequent spatial pulse shaper are used to generate laterally and axially tailored two-photon excited fluorescence profiles. The induced fluorescence is recorded for different polarization directions by utilizing the anisotropy of the dyes embedded in glycerol. We achieve an increased fluorescence contrast between different dyes by employing specific polarization shaped laser pulses and by taking polarization dependent emission into account. This versatile pulse shaping method is prospective for novel biophotonic imaging.

Graphical abstract

中文翻译：

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时空偏振光脉冲，用于双光子激发的荧光

摘要

我们目前为染料的两个光子激发的荧光同时空间和时间极化脉冲成形。时间脉冲整形器和随后的空间脉冲整形器用于生成横向和轴向定制的双光子激发的荧光轮廓。通过利用甘油中嵌入的染料的各向异性来记录针对不同偏振方向的诱导荧光。通过使用特定的偏振形状的激光脉冲并考虑到偏振相关的发射，我们实现了不同染料之间增强的荧光对比度。这种通用的脉冲整形方法有望用于新型生物光子成像。

图形概要