Contactless particle manipulation based on a thermal field has shown great potential for biological, medical, and materials science applications. However, thermal diffusion from a high-temperature area causes thermal damage to bio-samples. Besides, the permanent bonding of a sample chamber onto microheater substrates requires that the thermal field devices be non-disposable. These limitations impede use of the thermal manipulation approach. Here, a novel manipulation platform is proposed that combines microheaters and an area cooling system to produce enough force to steer sedimentary particles or cells and to limit the thermal diffusion. It uses the one-time fabricated motherboard and an exchangeable sample chamber that provides disposable use. Sedimentary objects can be steered to the bottom center of the thermal field by combined thermal convection and thermophoresis. Single particle or cell manipulation is realized by applying multiple microheaters in the platform. Results of a cell viability test confirmed the method's compatibility in biology fields. With its advantages of biocompatibility for live cells, operability for different sizes of particles and flexibility of platform fabrication, this novel manipulation platform has a high potential to become a powerful tool for biology research.

中文翻译：

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区域冷却可实现单电池的热定位和操纵

基于热场的非接触粒子处理在生物学，医学和材料科学应用中显示出巨大潜力。但是，从高温区域的热扩散会对生物样品造成热损害。此外，将样品腔室永久粘合到微型加热器基板上要求热场设备不可一次性使用。这些限制阻碍了热操纵方法的使用。在这里，提出了一种新颖的操纵平台，该平台结合了微型加热器和区域冷却系统，以产生足够的力来操纵沉积颗粒或细胞并限制热扩散。它使用一次性制造的主板和可更换的样品室，可一次性使用。通过热对流和热泳可以将沉积物引导至热场的底部中心。通过在平台中应用多个微型加热器，可以实现单个粒子或细胞的操作。细胞活力测试的结果证实了该方法在生物学领域的兼容性。凭借其与活细胞的生物相容性，对不同大小的颗粒的可操作性以及平台制造的灵活性等优点，这种新颖的操纵平台具有成为生物学研究的强大工具的巨大潜力。