The complexity of current nucleic acid isolation methods limits their use outside of the modern laboratory environment. Here, we describe a fast and affordable method to purify nucleic acids from animal, plant, viral and microbial samples using a cellulose-based dipstick. Nucleic acids can be purified by dipping in-house-made dipsticks into just three solutions: the extract (to bind the nucleic acids), a wash buffer (to remove impurities) and the amplification reaction (to elute the nucleic acids). The speed and simplicity of this method make it ideally suited for molecular applications, both within and outside the laboratory, including limited-resource settings such as remote field sites and teaching institutions. Detailed instructions for how to easily manufacture large numbers of dipsticks in house are provided. Using the instructions, readers can create more than 200 dipsticks in <30 min and perform dipstick-based nucleic acid purifications in 30 s.

当前核酸分离方法的复杂性限制了它们在现代实验室环境之外的使用。在这里，我们描述了一种使用基于纤维素的试纸从动物、植物、病毒和微生物样品中纯化核酸的快速且经济的方法。可以通过将自制试纸浸入三种溶液中来纯化核酸：提取物（用于结合核酸）、洗涤缓冲液（用于去除杂质）和扩增反应（用于洗脱核酸）。这种方法的速度和简单性使其非常适合实验室内外的分子应用，包括资源有限的环境，如远程现场和教学机构。提供了有关如何在内部轻松制造大量量油尺的详细说明。使用说明，