Abstract

Microbes that can be cultured and degrade petroleum are of particular interest for biotechnology such as bioremediation. This study aims to isolate and identify culturable petroleum-degrading bacteria and fungi from Brunei Darussalam, which has not previously been explored. A total of eight bacterial and nine fungal isolates that could degrade petroleum were obtained from petroleum-contaminated water or soil samples. DNA barcoding using 16S rRNA gene sequence identified five different bacterial genera which were Bacillus, Enterobacter, Micrococcus, Pseudoaltermonas and Pseudomonas. DNA barcoding using rRNA-ITS gene sequence identified nine different fungal taxa which were Aspergillus, Cladosporium, Exophiala, Flavodon, Hypocreales, Nectriaceae, Penicillium, Peniophora and Trichoderma. Biolog provided additional support to the identification of some isolates. This study is the first to report these unique microbes from Brunei Darussalam, which are of ecological and biotechnological value.

摘要

可以培养并降解石油的微生物对于生物技术（例如生物修复）特别感兴趣。这项研究旨在从文莱达鲁萨兰国分离和鉴定可培养的可降解石油的细菌和真菌，这以前没有进行过探索。从受石油污染的水或土壤样品中获得了总共八种细菌和九种可降解石油的真菌分离株。利用16S rRNA基因序列进行DNA条形码鉴定，鉴定到了5种不同的细菌属，分别为芽孢杆菌，肠杆菌，微球菌，假拟杆菌和假单胞菌。使用rRNA-ITS基因序列的DNA条形码识别了9种不同的真菌类群，即曲霉属，枝孢，外瓶，Flavodon，肉座菌目，Nectriaceae，青霉，隔孢伏革和木霉属。Biolog为鉴定某些分离株提供了额外的支持。这项研究首次报道了文莱达鲁萨兰国的这些独特微生物，具有生态和生物技术价值。