ABSTRACT

The antibacterial efficacy of ozonated sunflower oil against Enterococcus faecalis biofilm was evaluated comparing to gaseous ozone. Sixty disks of composite resin were distributed in sterile microtubes containing bacterial suspension of an antibiotic-resistant E. faecalis isolated from endodontic infection. After incubation for 24 hours at 37°C, six protocols were conducted (n = 10): group I, any treatment was performed; group II, treated with sunflower oil for 10 min; group III, treated with ozonized oil for 5 min; group IV, treated with ozonized oil for 10 min; group V, treated with ozone gas for 2 min; and group VI, treated with ozone gas for 4 min. The control groups I and II showed the highest numbers of colony-forming units (CFU)/mL, with significant differences in comparison to groups treated with ozone-based protocols. There was no significant statistical difference in the number of CFUs between ozone-treated groups. Ozonized oil for 10 min presented the lowest CFU/mL.

摘要

与气态臭氧相比，评估了臭氧化葵花籽油对粪肠球菌生物膜的抗菌功效。60 个复合树脂盘分布在无菌微管中，微管中含有抗生素抗性粪肠球菌的细菌悬浮液与牙髓感染分离。在 37°C 下孵育 24 小时后，进行了六种方案（n = 10）：I 组，进行任何治疗；II组，用葵花籽油处理10分钟；第三组，用臭氧油处理5分钟；IV组，用臭氧油处理10分钟；V组，臭氧处理2分钟；和 VI 组，用臭氧气体处理 4 分钟。对照组 I 和 II 显示出最高数量的菌落形成单位 (CFU)/mL，与使用基于臭氧的协议处理的组相比有显着差异。臭氧处理组之间的 CFU 数量没有显着的统计学差异。臭氧化油 10 分钟呈现最低的 CFU/mL。