Abstract

Brunfelsia uniflora (Pohl) D. Don roots have been widely used in folk medicine for treating inflammatory conditions. However, few studies have elucidated compounds that justify their traditional use. This study was conducted to characterize the phytochemical profile and evaluate the in vitro antioxidant capacity, and in vivo anti-inflammatory activity of extracts obtained from B. uniflora roots by comparing an herbal remedy (HR) with the crude hydroalcoholic extract (CHE). In the phytochemical analysis, scopoletin was identified as the marker compound. In quantitative analyses, CHE showed better results than HR. Furthermore, CHE had an effective anti-inflammatory activity. Animals treated with CHE (200 mg/kg) showed an 89.1% and a 73.8% reduction in edema volume after 1 hour of edema induction compared with those treated with negative control and positive control (indomethacin), respectively. These results show that B. uniflora root extracts have promising antioxidant and anti-inflammatory activities, thus corroborating their application in ethnomedicine.

摘要

Brunfelsia uniflora (Pohl) D. Don 根在民间医学中被广泛用于治疗炎症。然而，很少有研究阐明证明其传统用途合理的化合物。进行这项研究是为了表征植物化学特征并评估从B. uniflora 中获得的提取物的体外抗氧化能力和体内抗炎活性通过将草药 (HR) 与粗水醇提取物 (CHE) 进行比较来确定根。在植物化学分析中，东莨菪碱被鉴定为标记化合物。在定量分析中，CHE 显示出比 HR 更好的结果。此外，CHE具有有效的抗炎活性。与用阴性对照和阳性对照（消炎痛）治疗的动物相比，用 CHE (200 mg/kg) 治疗的动物在诱导水肿 1 小时后水肿体积分别减少 89.1% 和 73.8%。这些结果表明，B. uniflora根提取物具有良好的抗氧化和抗炎活性，从而证实了它们在民族医学中的应用。