Abstract

Information on the genetic structure of rice striped stem borer, Chilo suppressalis (Walker), is essential for an effective pest management program and can corporate with control program and prompt the modification of current control methods. In this study, the genetic structure of COI gene has been investigated for 18 populations of this pest in north of Iran. Chilo suppressalis specimens were collected from rice fields on rice and weeds in Guilan and Mazanderan provinces, at Caspian Sea coast at both seasonal activity (spring) and overwintering stage from May through October 2018 and identified using morphological characters. Genetic structure of 18 populations of the species was evaluated using polymerase chain reaction (PCR)-direct-sequencing of a fragment (869 bp) of mtDNA-COI gene. Results revealed that almost all specimens from different generations, hosts, and locations belong to a single haplotype very close to a South Korean haplotype. To the best our knowledge, it is the first comprehensive molecular typing of striped stem borer in both Northern provinces of the country and suggests a high gene flow among different populations of this pest in the region.

摘要

对水稻二化螟，遗传结构信息二化螟（沃克），是一个有效的害虫管理程序必需和能与控制程序和的电流控制方法的提示修饰的企业。在这项研究中，COI基因的遗传结构已在伊朗北部调查了该害虫的18个种群。抑制从2018年5月至2018年10月的季节性活动（春季）和越冬期，从里海和桂兰省和马赞德兰省稻米和杂草的稻田中收集标本，并利用形态特征进行鉴定。使用聚合酶链反应（PCR）-mtDNA-COI基因片段（869 bp）的直接测序评估了该物种18个种群的遗传结构。结果显示，几乎所有来自不同世代，宿主和位置的标本都属于非常接近韩国单体型的单个单体型。据我们所知，这是该国北部两个省的第一个全面的条纹茎bore虫分子分型，表明该地区该害虫的不同种群之间有很高的基因流量。