Abstract

580 microbial strains were isolated from the rhizosphere of the plants Cucurbita pepo L. and Xanthium strumarium grown on soil contaminated with dichlorodiphenyltrichloroethane (DDT) and its metabolites. During the cultivation, two bacterial strains were selected because of their ability to grow on media containing 0.5–5.0 mg L⁻¹ of dichlorodiphenyldichloroethylene (DDE) as the sole carbon source. They were identified as Bacillus vallismortis and Bacillus aryabhattai. Both of these species were shown to have a high capacity for the utilization of DDE – more than 90% of which was consumed after 21 days of cultivation. Laboratory experiments were carried out then to assess the possibility of using these strains for the decontamination of organochlorine pesticides (OCPs) contaminated soils. Inoculation of C. pepo and X. strumarium with our isolates B. vallismortis and B. aryabhattai resulted in a reduction of the pollutant stress to the plants as shown by an increase both in aboveground and in root biomass. The microorganisms enhanced the uptake and phytostabilization potential of C. pepo and X. strumarium and can be applied for the treatment of DDE contaminated soils.

抽象的

从在被二氯二苯基三氯乙烷（DDT）及其代谢物污染的土壤中生长的南瓜属（Cucurbita pepo L.）和黄原酸（Xanthium strumarium）植物的根际分离了580株微生物。在培养过程中，选择了两个细菌菌株，因为它们能够在含有0.5–5.0 mg L ^-1的二氯二苯基二氯乙烯（DDE）作为唯一碳源的培养基上生长。它们被鉴定为瓦氏芽孢杆菌和阿里亚巴泰芽孢杆菌。这两种物种均具有很高的DDE利用能力-种植21天后消耗了其中90％以上的DDE。然后进行了实验室实验，以评估使用这些菌株对有机氯农药（OCPs）污染的土壤进行去污的可能性。用我们的分离株vallismortis B. vallismortis和B. aryabhattai接种C. pepo和X. strumarium可以降低植物的污染物胁迫，这表现为地上和根系生物量的增加。微生物增强了C. pepo和X. strumarium的吸收和植物稳定潜力。 可以用于处理DDE污染的土壤。